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Literature summary for 2.4.1.19 extracted from

  • Jemli, S.; Ben Messaoud, E.; Ayadi-Zouari, D.; Naili, B.; Khemakhem, B.; Bejar, S.
    A beta-cyclodextrin glycosyltransferase from a newly isolated Paenibacillus pabuli US132 strain: purification, properties and potential use in bread-making (2007), Biochem. Eng. J., 34, 44-50.
No PubMed abstract available
Search for more literature for 2.4.1.19

Application

Application Comment Organism
food industry the enzyme can be used in the bread-baking process since its addition in the dough mix improved significantly the loaf volume and decreased the firmness of bread during storage Paenibacillus pabuli

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activates at 10 mM Paenibacillus pabuli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
70000
-
 1 * 70000, SDS-PAGE Paenibacillus pabuli

Organism

Organism UniProt Comment Textmining
Paenibacillus pabuli B1VC16 isolated from a Tunisian soil
-
Paenibacillus pabuli US132 B1VC16 isolated from a Tunisian soil
-

Purification (Commentary)

Purification (Comment) Organism
native CGTase 22.9fold to homogeneity by hydrophobic interaction chromatography and starch adsorption Paenibacillus pabuli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4000
-
 purified enzyme Paenibacillus pabuli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information production of beta-cyclodextrin from potato starch Paenibacillus pabuli ?
-
 ?
additional information production of beta-cyclodextrin from potato starch Paenibacillus pabuli US132 ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 70000, SDS-PAGE Paenibacillus pabuli

Synonyms

Synonyms Comment Organism
beta-cyclodextrin glycosyltransferase
-
 Paenibacillus pabuli
CGTase
-
 Paenibacillus pabuli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
 in absence of Ca2+ Paenibacillus pabuli
65
-
 in presence of 10 mM Ca2+ Paenibacillus pabuli

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 1 h, purified enzyme, 65% remaining activity Paenibacillus pabuli
55
-
 1 h, purified enzyme, 21% remaining activity Paenibacillus pabuli
60
-
 1 h, purified enzyme, no activity Paenibacillus pabuli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
 Paenibacillus pabuli

pH Range

pH Minimum pH Maximum Comment Organism
5.5 9
-
 Paenibacillus pabuli