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Literature summary for 2.4.1.19 extracted from

  • Kaulpiboon, J.; Pongsawasdi, P.; Zimmermann, W.
    Molecular imprinting of cyclodextrin glycosyltransferases from Paenibacillus sp. A11 and Bacillus macerans with gamma-cyclodextrin (2007), FEBS J., 274, 1001-1010.
    View publication on PubMed

General Stability

General Stability Organism
more than 80% of the initial immobilized and crosslinked imprinted CGTase activity is retained for up to five cycles of synthesis reactions Paenibacillus macerans
more than 80% of the initial immobilized and crosslinked imprinted CGTase activity is retained for up to five cycles of synthesis reactions Paenibacillus sp.
the immobilized and CD8-crosslinked imprinted CGTase shows 15% higher stability in phosphate buffer containing up to 50% ethanol or cyclohexane compared to the native enzyme Paenibacillus macerans
the immobilized and CD8-crosslinked imprinted CGTase shows 30% higher stability in phosphate buffer containing up to 50% ethanol or cyclohexane compared to the native enzyme Paenibacillus sp.
the temperature stability of the immobilized and crosslinked imprinted CGTase at 60°C is considerably higher than that of the native enzyme Paenibacillus macerans
the temperature stability of the immobilized and crosslinked imprinted CGTase at 60°C is considerably higher than that of the native enzyme Paenibacillus sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
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additional information
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Paenibacillus macerans
additional information
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additional information
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Paenibacillus sp.

Organism

Organism UniProt Comment Textmining
Paenibacillus macerans
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-
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Paenibacillus sp.
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-
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Paenibacillus sp. A11
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-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
starch to manipulate the product specificity of the Paenibacillus sp. A11 and Bacillus macerans cyclodextrin glycosyltransferases towards the preferential formation of gamma-cyclodextrin (CD8), crosslinked imprinted protein of cyclodextrin glycosyltransferase is prepared by applying enzyme imprinting and immobilization methodologies. The native enzyme produces CD6:CD7:CD8:CD9 ratios of 15:65:20:0 at 40°C. The size of the synthesis products formed by the crosslinked imprinted cyclodextrin glycosyltransferases is shifted towards CD8 and CD9, and the overall cyclodextrin yield is increased by 12% compared to the native enzymes Paenibacillus sp. cyclodextrin
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?
starch to manipulate the product specificity of the Paenibacillus sp. A11 and Bacillus macerans cyclodextrin glycosyltransferases towards the preferential formation of gamma-cyclodextrin (CD8), crosslinked imprinted protein of cyclodextrin glycosyltransferase is prepared by applying enzyme imprinting and immobilization methodologies. The native enzyme produces CD6:CD7:CD8:CD9 ratios of 43:36:21:0 at 40°C. The size of the synthesis products formed bythe crosslinked imprinted cyclodextrin glycosyltransferases is shifted towards CD8 and CD9, and the overall cyclodextrin yield is increased by 12% compared to the native enzymes Paenibacillus macerans cyclodextrin
-
?
starch to manipulate the product specificity of the Paenibacillus sp. A11 and Bacillus macerans cyclodextrin glycosyltransferases towards the preferential formation of gamma-cyclodextrin (CD8), crosslinked imprinted protein of cyclodextrin glycosyltransferase is prepared by applying enzyme imprinting and immobilization methodologies. The native enzyme produces CD6:CD7:CD8:CD9 ratios of 15:65:20:0 at 40°C. The size of the synthesis products formed by the crosslinked imprinted cyclodextrin glycosyltransferases is shifted towards CD8 and CD9, and the overall cyclodextrin yield is increased by 12% compared to the native enzymes Paenibacillus sp. A11 cyclodextrin
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?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40 50 native enzyme Paenibacillus sp.
60
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native enzyme Paenibacillus macerans

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 70 30°C: about 45% of maximal activity, 70°C: about 80% of maximal activity, native enzyme Paenibacillus macerans
30 70 30°C: about 450% of maximal activity, 90°C: about 50% of maximal activity, native enzyme Paenibacillus sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
pH 6.0, 30 min, native enzyme shows about 10% loss of activity. Iimmobilized and crosslinked imprinted CGTase are stable Paenibacillus sp.
60
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pH 6.0, 30 min, native enzyme shows about 10% loss of activity. Immobilized and crosslinked imprinted CGTase are stable Paenibacillus macerans
70
-
pH 6.0, 30 min, native enzyme is completely inactivated. Immobilized and crosslinked imprinted CGTase shows about 50% loss of activity Paenibacillus sp.
70
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pH 6.0, 30 min, native enzyme shows about 50% loss of activity. Immobilized and crosslinked imprinted CGTase show about 15% loss of activity Paenibacillus macerans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
native enzyme Paenibacillus macerans
6 8 native enzyme Paenibacillus sp.

pH Range

pH Minimum pH Maximum Comment Organism
5 9 pH 5.0: about 50% of maximal activity, pH 9.0: about 55% of maximal activity, native enzyme Paenibacillus sp.
5 7 pH 5.0: about 70% of maximal activity, pH 9.0: about 70% of maximal activity, native enzyme Paenibacillus macerans

pH Stability

pH Stability pH Stability Maximum Comment Organism
additional information
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the immobilized and the crosslinked imprinted CGTase are more stable than the native enzyme in the pH ranges from 3 to 6 and 8 to 11 Paenibacillus sp.
additional information
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the immobilized and the crosslinked imprinted CGTase shows higher stability in the ranges from pH 3 to 7 and from pH 9 to 11 Paenibacillus macerans