Crystallization (Comment) | Organism |
---|---|
hanging-drop vapour-diffusion method, crystal structure of native and acarbose-complexed mutant CGTase F283L and F283Y | Bacillus sp. (in: Bacteria) |
Protein Variants | Comment | Organism |
---|---|---|
F283L | starch degrading activity is similar to that of wild-type enzyme between the acidic and neutral pH ranges but decreases to 10% at pH 10.0. The pH-value of half-maximal activity at basic pH side is shifted to 8.6 from 10.0 of the wild-type. 23%-67% decrease in KM-value for 3-ketobutylidene-beta-2-chloro-4-nitrophenylmalto-pentaoside in the disproportionation reaction. The turnover-number for the disproportionating reaction at various pH conditions decreases to 1.6% to 4.4% compared with those of wild-type enzyme | Bacillus sp. (in: Bacteria) |
F283Y | mutation decreases the enzymatic activity in the basic pH range | Bacillus sp. (in: Bacteria) |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus sp. (in: Bacteria) | - |
1011 | - |
Bacillus sp. (in: Bacteria) 1011 | - |
1011 | - |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4 | - |
starch degrading activity, wild-type enzyme | Bacillus sp. (in: Bacteria) |
7 | - |
starch degrading activity, mutant enzyme F283L | Bacillus sp. (in: Bacteria) |