General Stability | Organism |
---|---|
Ca2+, 20 mM, increases thermal stability | Bacillus sp. (in: Bacteria) |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CoCl2 | 1 mM, complete inhibition | Bacillus sp. (in: Bacteria) | |
CuSO4 | 1 mM, complete inhibition | Bacillus sp. (in: Bacteria) | |
EDTA | 1 mM, 83% inhibition | Bacillus sp. (in: Bacteria) | |
FeCl3 | 1 mM, 70% inhibition | Bacillus sp. (in: Bacteria) | |
FeSO4 | 1 mM, complete inhibition | Bacillus sp. (in: Bacteria) | |
K2SO4 | 1 mM, 54% inhibition | Bacillus sp. (in: Bacteria) | |
MgCl2 | 1 mM, 53% inhibition | Bacillus sp. (in: Bacteria) | |
MgSO4 | 1 mM, 65% inhibition | Bacillus sp. (in: Bacteria) | |
NaCl | 1 mM, 28% inhibition | Bacillus sp. (in: Bacteria) | |
NaNO3 | 1 mM, 54% inhibition | Bacillus sp. (in: Bacteria) | |
SDS | 1 mM, 55% inhibition | Bacillus sp. (in: Bacteria) | |
ZnCl2 | 1 mM, 73% inhibition | Bacillus sp. (in: Bacteria) | |
ZnSO4 | 1 mM, complete inhibition | Bacillus sp. (in: Bacteria) |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
FeCl2 | 1 mM, 89.3% activation | Bacillus sp. (in: Bacteria) |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
75000 | - |
1 * 75000, SDS-PAGE | Bacillus sp. (in: Bacteria) |
79000 | - |
gel filtration | Bacillus sp. (in: Bacteria) |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus sp. (in: Bacteria) | - |
G1 | - |
Bacillus sp. (in: Bacteria) G1 | - |
G1 | - |
Purification (Comment) | Organism |
---|---|
- |
Bacillus sp. (in: Bacteria) |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
soluble starch + glycosyl acceptor | tapioca starch is the best substrate | Bacillus sp. (in: Bacteria) | beta-cyclodextrin + gamma-cyclodextrin | production ratio of beta-cyclodextrin to gamma-cyclodextrin is 0.11/0.89 after 24 h at 60°C, without presence of any selective agents | ? | |
soluble starch + glycosyl acceptor | tapioca starch is the best substrate | Bacillus sp. (in: Bacteria) G1 | beta-cyclodextrin + gamma-cyclodextrin | production ratio of beta-cyclodextrin to gamma-cyclodextrin is 0.11/0.89 after 24 h at 60°C, without presence of any selective agents | ? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 75000, SDS-PAGE | Bacillus sp. (in: Bacteria) |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Bacillus sp. (in: Bacteria) |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 70 | 50°C: about 15% of maximal activity, 70°C: about 20% of maximal activity | Bacillus sp. (in: Bacteria) |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
pH 6.0, 30 min, stable up to | Bacillus sp. (in: Bacteria) |
70 | - |
in presence of 20 mM Ca2+, stable up to | Bacillus sp. (in: Bacteria) |
76 | - |
pH 6.0, 30 min, 50% loss of activity | Bacillus sp. (in: Bacteria) |
80 | - |
pH 6.0, 30 min, 86% loss of activity | Bacillus sp. (in: Bacteria) |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Bacillus sp. (in: Bacteria) |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 7 | pH 5.0: about 25% of maximal activity, pH 7.0: about 60% of maximal activity, the enzyme does not show any activity at pH 4.0 and at pH 10.0 | Bacillus sp. (in: Bacteria) |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7 | 9 | 60°C, 30 min, stable | Bacillus sp. (in: Bacteria) |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Bacillus sp. (in: Bacteria) | - |
- |
8.8 |