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Literature summary for 2.4.1.19 extracted from

  • Sian, H.K.; Said, M.; Hassan, O.; Kamaruddin, K.; Ismail, A.F.; Rahman, R.A.; Mahmood, N.A.N.; Illias, R.M.
    Purification and characterization of cyclodextrin glucanotransferase from alkalophilic Bacillus sp. G1 (2004), Proc. Biochem., 40, 1101-1111.
No PubMed abstract available

General Stability

General Stability Organism
Ca2+, 20 mM, increases thermal stability Bacillus sp. (in: Bacteria)

Inhibitors

Inhibitors Comment Organism Structure
CoCl2 1 mM, complete inhibition Bacillus sp. (in: Bacteria)
CuSO4 1 mM, complete inhibition Bacillus sp. (in: Bacteria)
EDTA 1 mM, 83% inhibition Bacillus sp. (in: Bacteria)
FeCl3 1 mM, 70% inhibition Bacillus sp. (in: Bacteria)
FeSO4 1 mM, complete inhibition Bacillus sp. (in: Bacteria)
K2SO4 1 mM, 54% inhibition Bacillus sp. (in: Bacteria)
MgCl2 1 mM, 53% inhibition Bacillus sp. (in: Bacteria)
MgSO4 1 mM, 65% inhibition Bacillus sp. (in: Bacteria)
NaCl 1 mM, 28% inhibition Bacillus sp. (in: Bacteria)
NaNO3 1 mM, 54% inhibition Bacillus sp. (in: Bacteria)
SDS 1 mM, 55% inhibition Bacillus sp. (in: Bacteria)
ZnCl2 1 mM, 73% inhibition Bacillus sp. (in: Bacteria)
ZnSO4 1 mM, complete inhibition Bacillus sp. (in: Bacteria)

Metals/Ions

Metals/Ions Comment Organism Structure
FeCl2 1 mM, 89.3% activation Bacillus sp. (in: Bacteria)

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
75000
-
1 * 75000, SDS-PAGE Bacillus sp. (in: Bacteria)
79000
-
gel filtration Bacillus sp. (in: Bacteria)

Organism

Organism UniProt Comment Textmining
Bacillus sp. (in: Bacteria)
-
G1
-
Bacillus sp. (in: Bacteria) G1
-
G1
-

Purification (Commentary)

Purification (Comment) Organism
-
Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
soluble starch + glycosyl acceptor tapioca starch is the best substrate Bacillus sp. (in: Bacteria) beta-cyclodextrin + gamma-cyclodextrin production ratio of beta-cyclodextrin to gamma-cyclodextrin is 0.11/0.89 after 24 h at 60°C, without presence of any selective agents ?
soluble starch + glycosyl acceptor tapioca starch is the best substrate Bacillus sp. (in: Bacteria) G1 beta-cyclodextrin + gamma-cyclodextrin production ratio of beta-cyclodextrin to gamma-cyclodextrin is 0.11/0.89 after 24 h at 60°C, without presence of any selective agents ?

Subunits

Subunits Comment Organism
monomer 1 * 75000, SDS-PAGE Bacillus sp. (in: Bacteria)

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
Bacillus sp. (in: Bacteria)

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
50 70 50°C: about 15% of maximal activity, 70°C: about 20% of maximal activity Bacillus sp. (in: Bacteria)

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
pH 6.0, 30 min, stable up to Bacillus sp. (in: Bacteria)
70
-
in presence of 20 mM Ca2+, stable up to Bacillus sp. (in: Bacteria)
76
-
pH 6.0, 30 min, 50% loss of activity Bacillus sp. (in: Bacteria)
80
-
pH 6.0, 30 min, 86% loss of activity Bacillus sp. (in: Bacteria)

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
Bacillus sp. (in: Bacteria)

pH Range

pH Minimum pH Maximum Comment Organism
5 7 pH 5.0: about 25% of maximal activity, pH 7.0: about 60% of maximal activity, the enzyme does not show any activity at pH 4.0 and at pH 10.0 Bacillus sp. (in: Bacteria)

pH Stability

pH Stability pH Stability Maximum Comment Organism
7 9 60°C, 30 min, stable Bacillus sp. (in: Bacteria)

pI Value

Organism Comment pI Value Maximum pI Value
Bacillus sp. (in: Bacteria)
-
-
8.8