Protein Variants | Comment | Organism |
---|---|---|
additional information | engineering cyclodextrin glycosyltransferase into a starch hydrolase with a high exospecificity. the cyclodextrin product specificity can be changed into linear product specificity, by introducing a five-residue insertion mutation at the donor substrate binding subsites. The CGTase mutants remain clearly different from the maltogenic alpha-amylase, as they have much lower hydrolytic activities, they form linear products of variable sizes and they retain a low cyclodextrin forming activity, whereas maltogenic alpha-amylases produce primarily maltose. The five-residue insertion, concomitantly, strongly enhances the exo-specificity of CGTase | Thermoanaerobacterium thermosulfurigenes |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermoanaerobacterium thermosulfurigenes | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
starch + glycosyl acceptor | the cyclodextrin product specificity can be changed into linear product specificity, by introducing a five-residue insertion mutation at the donor substrate binding subsites. The CGTase mutants remain clearly different from the maltogenic alpha-amylase, as they have much lower hydrolytic activities, they form linear products of variable sizes and they retain a low cyclodextrin forming activity, whereas maltogenic alpha-amylases produce primarily maltose. The five-residue insertion, concomitantly, strongly enhances the exo-specificity of CGTase | Thermoanaerobacterium thermosulfurigenes | cyclodextrin | - |
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Synonyms | Comment | Organism |
---|---|---|
CGTase | - |
Thermoanaerobacterium thermosulfurigenes |