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Literature summary for 2.4.1.19 extracted from

  • Martins, R.F.; Hatti-Kaul, R.
    Bacillus agaradhaerens LS-3C cyclodextrin glycosyltransferase: activity and stability features (2003), Enzyme Microb. Technol., 33, 819-827.
No PubMed abstract available
Search for more literature for 2.4.1.19

Activating Compound

Activating Compound Comment Organism Structure
PEG 3000 10% w/v, increases cyclodextrin production Salipaludibacillus agaradhaerens
sorbitol 1 mM, increases cyclodextrin production Salipaludibacillus agaradhaerens

General Stability

General Stability Organism
enzyme stability is greatly enhanced with sorbitol Salipaludibacillus agaradhaerens

Inhibitors

Inhibitors Comment Organism Structure
beta-cyclodextrin inhibits cyclization Salipaludibacillus agaradhaerens
maltose inhibits cyclization Salipaludibacillus agaradhaerens

Organism

Organism UniProt Comment Textmining
Salipaludibacillus agaradhaerens
-
 LS-3C
-
Salipaludibacillus agaradhaerens LS-3C
-
 LS-3C
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
maltodextrin + glycosyl acceptor displays unusually high amylolytic activity in relation to the cyclization activity. Disproportionation activity of the CGTase is optimal with maltose as the acceptor substrate. Cyclization reaction and beta-cyclodextrin formation are significantly promoted in the presence of CaCl2. The salt allows the cyclization reaction to be performed at higher temperature Salipaludibacillus agaradhaerens beta-cyclodextrin + alpha-cyclodextrin + gamma-cyclodextrin the product of cyclization reaction is predominantly beta-cyclodextrin along with alpha-cyclodextrin as a minor product. The CDs profile is influenced by the reaction conditions. At pH 10, alpha-cyclodextrin is replaced by gamma-cyclodextrin formation ?
maltodextrin + glycosyl acceptor displays unusually high amylolytic activity in relation to the cyclization activity. Disproportionation activity of the CGTase is optimal with maltose as the acceptor substrate. Cyclization reaction and beta-cyclodextrin formation are significantly promoted in the presence of CaCl2. The salt allows the cyclization reaction to be performed at higher temperature Salipaludibacillus agaradhaerens LS-3C beta-cyclodextrin + alpha-cyclodextrin + gamma-cyclodextrin the product of cyclization reaction is predominantly beta-cyclodextrin along with alpha-cyclodextrin as a minor product. The CDs profile is influenced by the reaction conditions. At pH 10, alpha-cyclodextrin is replaced by gamma-cyclodextrin formation ?