Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.4.1.19 extracted from

  • Yamamoto, T.; Shiraki, K.; Fujiwara, S.; Takagi, M.; Fukui, K.; Imanaka, T.
    In vitro heat effect on functional and conformational changes of cyclodextrin glucanotransferase from hyperthermophilic archaea (1999), Biochem. Biophys. Res. Commun., 265, 57-61.
    View publication on PubMed
Search for more literature for 2.4.1.19

Cloned(Commentary)

Cloned (Comment) Organism
-
 Thermococcus sp.
cgtA gene cloned and expressed in Escherichia coli JM109 Thermococcus sp.

Organism

Organism UniProt Comment Textmining
Geobacillus stearothermophilus
-
-
-
Niallia circulans
-
-
-
Niallia circulans
-
 no. 8
-
Niallia circulans 251
-
-
-
Niallia circulans 8
-
 no. 8
-
Thermoanaerobacterium thermosulfurigenes
-
 EM1
-
Thermoanaerobacterium thermosulfurigenes EM1
-
 EM1
-
Thermococcus sp.
-
 B1001
-
Thermococcus sp. Q9UWN2 B1001, DNA sequence of CGTase gene, EMBL/GenBank/DDBJ database accession No.
-
Thermococcus sp. B1001
-
 B1001
-

Purification (Commentary)

Purification (Comment) Organism
natural and recombinant enzyme Thermococcus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
soluble starch + glycosyl acceptor
-
 Thermococcus sp. alpha-cyclodextrin
-
 r
soluble starch + glycosyl acceptor
-
 Thermococcus sp. B1001 alpha-cyclodextrin
-
 r
starch
-
 Thermococcus sp. beta-cyclodextrin
-
 ?
starch
-
 Thermococcus sp. B1001 beta-cyclodextrin
-
 ?

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 107
-
 Thermococcus sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
80 110 above 80°C activity increases by heat treatment, enzyme is irreversibly unfolded above 110°C Thermococcus sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1
-
 starch unheated CGTase Thermococcus sp.
1.33
-
 starch heated CGTase Thermococcus sp.