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Literature summary for 2.4.1.19 extracted from
- Cyclodextrin glucanotransferase from Klebsiella pneumoniae. 1. Formation, purification and properties of the enzyme from Klebsiella pneumoniae M 5 al (1977), Arch. Microbiol., 111, 271-282.
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Paenibacillus macerans | - |
- |
| extracellular | - |
Klebsiella oxytoca | - |
- |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Klebsiella oxytoca | - |
formerly Klebsiella pneumoniae M5a1 | - |
| Klebsiella pneumoniae | - |
M5 | - |
| Klebsiella pneumoniae M5 | - |
M5 | - |
| Paenibacillus macerans | - |
- |
- |
| Priestia megaterium | - |
No5 | - |
| Priestia megaterium No5 | - |
No5 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Klebsiella pneumoniae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alpha-1,4-glucan + glycosyl acceptor | - |
Priestia megaterium | cyclohexaamylose + cycloheptaamylose + cyclooctaamylose | product ratio 1: 2.4: 1 | r |  |
| alpha-1,4-glucan + glycosyl acceptor | - |
Priestia megaterium No5 | cyclohexaamylose + cycloheptaamylose + cyclooctaamylose | product ratio 1: 2.4: 1 | r | |
| amylopectin + glycosyl acceptor | - |
Klebsiella oxytoca | cycloheptaamylose + cyclohexaamylose + exo-branched cyclohexaamylose | - |
r | |
| cycloheptaamylose + glycosyl acceptor | - |
Klebsiella oxytoca | ? | - |
r | |
| cyclohexaamylose + glycosyl acceptor | - |
Klebsiella oxytoca | ? | - |
r | |
| soluble starch + H2O | potato starch | Klebsiella pneumoniae | cyclodextrins | - |
? | |
| soluble starch + H2O | potato starch | Paenibacillus macerans | cyclodextrins | - |
? | |
| soluble starch + H2O | potato starch | Klebsiella pneumoniae M5 | cyclodextrins | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | 55 | stable up to 45°C, 13.4% activity at 50°C, no activity above 55°C | Klebsiella oxytoca |
| 80 | - |
15 min at 80°C, after incubation for 12 h reactivation to a little restactivity | Klebsiella oxytoca |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.2 | - |
strain M5 a1 | Klebsiella oxytoca |
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Release 2023.1 (January 2023)
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