Cloned (Comment) | Organism |
---|---|
expresssion of recombinant protein in the CGSC Escherichia coli cell line 4997, which lacks UDP-glucose pyrophosphorylase activity | Oryctolagus cuniculus |
General Stability | Organism |
---|---|
concentrated to 8 mg/ml, and 100 microl aliquots are flash frozen in liquid nitrogen and stored at -80°C. Glycogenin stored in this manner is stable for at least 2 years. | Oryctolagus cuniculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryctolagus cuniculus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
nickel-NTA column and Q-Sepharose column | Oryctolagus cuniculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
muscle | - |
Oryctolagus cuniculus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-alpha-D-glucose + glycogenin | - |
Oryctolagus cuniculus | additional information | after 60 min of incubation the glycogenin molecules possess an average glucosyl chain length of 11.3 residues | ? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | the catalytic effectiveness of glycogenin falls off dramatically as the average glucosyl chain length increases | Oryctolagus cuniculus | |
0.0053 | - |
UDP-glucose | glucosylated protein, 14C-glucose incorporation assay | Oryctolagus cuniculus | |
0.0083 | - |
UDP-glucose | glucosylated protein, 4-10 interval, mass sprectrometry assay | Oryctolagus cuniculus | |
0.0083 | - |
UDP-glucose | non-glucosylated protein, 14C-glucose incorporation assay | Oryctolagus cuniculus | |
0.0183 | - |
UDP-glucose | non-glucosylated protein, mass sprectrometry assay | Oryctolagus cuniculus |