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Literature summary for 2.4.1.186 extracted from

  • Viskupic, E.; Cao, Y.; Zhang, W.; Cheng, C.; DePaoli-Roach, A.A.; Roach, P.J.
    Rabbit skeletal muscle glycogenin. Molecular cloning and production of fully functional protein in Escherichia coli (1992), J. Biol. Chem., 267, 25759-25763.
    View publication on PubMed
Search for more literature for 2.4.1.186

Cloned(Commentary)

Cloned (Comment) Organism
expression of mutant Y194F in Escherichia coli Oryctolagus cuniculus
functional expression of the wild-type enzyme in Escherichia coli Oryctolagus cuniculus

Protein Variants

Protein Variants Comment Organism
Y194F exchange of glucose attachment site, no autoglucosylation activity Rattus norvegicus
Y194F exchange of glucose attachment site, no autoglucosylation activity Oryctolagus cuniculus

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+
-
 Oryctolagus cuniculus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-
Oryctolagus cuniculus
-
-
-
Rattus norvegicus
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein 1 single glucose attachment site at tyrosine-194 Oryctolagus cuniculus
glycoprotein autoglycosylation Rattus norvegicus
glycoprotein autoglycosylation Oryctolagus cuniculus

Reaction

Reaction Comment Organism Reaction ID
UDP-alpha-D-glucose + glycogenin = UDP + alpha-D-glucosylglycogenin initiation of glycogen biosynthesis is a 2-step mechanism, requiring first the covalent attachment of a glucose residue to Tyr-194 of glycogenin and then elongation to form an oligosaccharide chain Oryctolagus cuniculus
a
UDP-alpha-D-glucose + glycogenin = UDP + alpha-D-glucosylglycogenin highly conserved protein Rattus norvegicus
a
UDP-alpha-D-glucose + glycogenin = UDP + alpha-D-glucosylglycogenin highly conserved protein Bos taurus
a
UDP-alpha-D-glucose + glycogenin = UDP + alpha-D-glucosylglycogenin highly conserved protein Oryctolagus cuniculus
a

Source Tissue

Source Tissue Comment Organism Textmining
kidney
-
 Rattus norvegicus
-
kidney
-
 Bos taurus
-
kidney low content Oryctolagus cuniculus
-
liver
-
 Rattus norvegicus
-
liver very low content Oryctolagus cuniculus
-
skeletal muscle
-
 Rattus norvegicus
-
skeletal muscle
-
 Bos taurus
-
skeletal muscle
-
 Oryctolagus cuniculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-glucose + glycogenin autoglycosylation reaction Rattus norvegicus UDP + glucosylated glycogenin
-
 ?
UDP-glucose + glycogenin autoglycosylation reaction Bos taurus UDP + glucosylated glycogenin
-
 ?
UDP-glucose + glycogenin autoglycosylation reaction Oryctolagus cuniculus UDP + glucosylated glycogenin glucose molecule is attached to Tyr-194 ?