Cloned (Comment) | Organism |
---|---|
recombinant expression of His-tagged isozymes rBE2 and rBE3 in Escherichia coli strain BL21(DE3) and in strain DELTAglgCAP with BL21(DE3) background | Arabidopsis thaliana |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged isozymes rBE2 and rBE3 from Escherichia coli strains by nickel affinity chromatography | Arabidopsis thaliana |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Arabidopsis thaliana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Starch synthase I activity in native PAGE without addition of glucans is dependent on at least one of the two branching enzyme isoforms active in Arabidopsis leaves. This interaction is most likely not based on a physical association of the enzymes. Chain length distribution patterns of the formed glucans are irrespective of enzyme isoforms origin and still independent of assay conditions. All starch synthase isoforms I-IV are able to interact with branching enzymes and form branched glucans, product analysis, overview | Arabidopsis thaliana | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
SBE | - |
Arabidopsis thaliana |
starch branching enzyme | - |
Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Arabidopsis thaliana |
General Information | Comment | Organism |
---|---|---|
metabolism | starch synthase and branching enzyme establish the two glycosidic linkages existing in starch. Both enzymes exist as several isoforms. Starch synthase I activity in native PAGE without addition of glucans is dependent on at least one of the two branching enzyme isoforms active in Arabidopsis leaves. This interaction is most likely not based on a physical association of the enzymes. All starch synthase isoforms I-IV are able to interact with branching enzymes and form branched glucans | Arabidopsis thaliana |