Activating Compound | Comment | Organism | Structure |
---|---|---|---|
NaCl | concentration of 0.5 - 1 M NaCl | Rhodothermus marinus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
DMSO | about 22.9% decreased enzyme activity, 10% | Rhodothermus marinus | |
EDTA | about 44.2% decreased enzyme activity, 5 mM | Rhodothermus marinus | |
ethanol | about 57.2% decreased enzyme activity, 10% | Rhodothermus marinus | |
methanol | about 45.2% decreased enzyme activity, 10% | Rhodothermus marinus | |
NaCl | concetration of 2-4 M NaCl | Rhodothermus marinus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Rhodothermus marinus | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | about 2.5% decreased enzyme activity, 5 mM | Rhodothermus marinus | |
Ca2+ | about 3.6% decreased enzyme activity, 5 mM | Rhodothermus marinus | |
Cu2+ | about 16.4% decreased enzyme activity, 5 mM | Rhodothermus marinus | |
Fe2+ | about 58.3% decreased enzyme activity, 5 mM | Rhodothermus marinus | |
Li+ | - |
Rhodothermus marinus | |
Mg2+ | 15% increased enzyme activity, 5 mM | Rhodothermus marinus | |
Mn2+ | about 53.5% decreased enzyme activity, 5 mM | Rhodothermus marinus | |
Zn2+ | about 61.8% decreased enzyme activity, 5 mM | Rhodothermus marinus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodothermus marinus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate, Q-Sepharose column, ultrafiltration, gel filtration with Superdex 200 column, native polyacrylamide gel elektrophoresis | Rhodothermus marinus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
amylose | dinitrosalicylate method, assay at pH 6.0, 70°C | Rhodothermus marinus | ? | - |
? | |
Maltohexaose | dinitrosalicylate method, assay at pH 6.0, 70°C | Rhodothermus marinus | ? | - |
? | |
maltopentaose | dinitrosalicylate method, assay at pH 6.0, 70°C | Rhodothermus marinus | ? | - |
? | |
Pullulan | dinitrosalicylate method, assay at pH 6.0, 70°C | Rhodothermus marinus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
RMEBE | - |
Rhodothermus marinus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
- |
Rhodothermus marinus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 90 | - |
Rhodothermus marinus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
75 | - |
fully active after 100 min incubation, half-life time at 80°C 73.7 min, half-life time at 85°C 16.7 min | Rhodothermus marinus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Rhodothermus marinus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3 | 9 | - |
Rhodothermus marinus |