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Literature summary for 2.4.1.18 extracted from

  • Hamada, S.; Nozaki, K.; Ito, H.; Yoshimoto, Y.; Yoshida, H.; Hiraga, S.; Onodera, S.; Honma, M.; Takeda, Y.; Matsui, H.
    Two starch-branching-enzyme isoforms occur in different fractions of developing seeds of kidney bean (2001), Biochem. J., 359, 23-34.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.4.1.18

Activating Compound

Activating Compound Comment Organism Structure
citrate 0.3 M, more than 3fold stimulation of activity of isoenzyme SBE2, no effect on activity of isoenzyme SBE1 Phaseolus vulgaris

Cloned(Commentary)

Cloned (Comment) Organism
isoenzymes PySBE1 and PySBE2, expression in Escherichia coli Phaseolus vulgaris

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Km-value for amylose, isoenzyme SBE2: 1.27 mg/ml. Km-value for amylose, isoenzyme SBE1: 0.46 mg/ml Phaseolus vulgaris

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble SBE2 is present in the soluble fraction, SBE1 is associated with the starch granule fraction Phaseolus vulgaris
-
-
starch grain SBE2 is present in the soluble fraction, SBE1 is associated with the starch granule fraction Phaseolus vulgaris 43036
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
81932
-
 1 * 81932, isoenzyme SBE2, calculation from nucleotide sequence Phaseolus vulgaris
82000
-
 1 * 82000, isoenzyme SBE2, SDS-PAGE Phaseolus vulgaris
88590
-
 1 * 88590, isoenzyme SBE1, calculation from nucleotide sequence Phaseolus vulgaris
100000
-
 1 * 100000, isoenzyme SBE1, SDS-PAGE Phaseolus vulgaris

Organism

Organism UniProt Comment Textmining
Phaseolus vulgaris
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining

Subunits

Subunits Comment Organism
monomer 1 * 100000, isoenzyme SBE1, SDS-PAGE Phaseolus vulgaris
monomer 1 * 88590, isoenzyme SBE1, calculation from nucleotide sequence Phaseolus vulgaris
monomer 1 * 82000, isoenzyme SBE2, SDS-PAGE Phaseolus vulgaris
monomer 1 * 81932, isoenzyme SBE2, calculation from nucleotide sequence Phaseolus vulgaris

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 pH 8.0, 15 min, stable up to, enzyme form KBE1 Phaseolus vulgaris
50
-
 pH 8.0, 15 min, stable up to, enzyme form KBE2 Phaseolus vulgaris

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Phaseolus vulgaris

pH Stability

pH Stability pH Stability Maximum Comment Organism
7 9 4°C, 16 h, less than 10% loss of activity, isoenzyme SBE2 Phaseolus vulgaris
7 8.5 4°C, 16 h, less than 10% loss of activity, isoenzyme SBE1 Phaseolus vulgaris