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Literature summary for 2.4.1.175 extracted from
- Detection and characterization of UDP-GalNAc: chondroitin N-acetylgalactosaminyltransferase in bovine serum using a simple assay method (1995), J. Biochem., 117, 1083-1087.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.05 | - |
UDP-N-acetyl-beta-D-galactosamine | - |
Bos taurus |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Bos taurus | 5829 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mn2+ | required, maximal at about 10 mM | Bos taurus | |
| additional information | Mg2+, Cu2+, Ca2+, Zn2+, Ba2+ do not effect the enzyme activity | Bos taurus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-N-acetyl-alpha-D-galactosamine + chondroitin oligosaccharide | Bos taurus | involved in chondroitin biosynthesis | UDP + N-acetyl-beta-D-galactosaminyl chondroitin oligosaccharide | - |
? | |
| UDP-N-acetyl-alpha-D-galactosamine + chondroitin sulfate oligosaccharide | Bos taurus | involved in chondroitin sulfate biosynthesis: elongation of growing chondroitin sulfate chain | UDP + N-acetyl-beta-D-galactosaminyl chondroitin sulfate oligosaccharide | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
calf | - |
| Bos taurus | - |
adult | - |
| Bos taurus | - |
fetal | - |
| Rattus norvegicus | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| serum | - |
Rattus norvegicus | - |
| serum | - |
Bos taurus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Rattus norvegicus |
| additional information | - |
assay method development | Bos taurus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | chondroitin sulfates A, C, D, and E, and dermatan sulfate are poor acceptor substrates | Bos taurus | ? | - |
? | |
| UDP-N-acetyl-alpha-D-galactosamine + chondroitin oligosaccharide | involved in chondroitin biosynthesis | Bos taurus | UDP + N-acetyl-beta-D-galactosaminyl chondroitin oligosaccharide | - |
? | |
| UDP-N-acetyl-alpha-D-galactosamine + chondroitin sulfate oligosaccharide | involved in chondroitin sulfate biosynthesis: elongation of growing chondroitin sulfate chain | Bos taurus | UDP + N-acetyl-beta-D-galactosaminyl chondroitin sulfate oligosaccharide | - |
? | |
| UDP-N-acetyl-beta-D-galactosamine + chondroitin | - |
Bos taurus | ? | - |
? | |
| UDP-N-acetyl-D-galactosamine + chondroitin oligosaccharide | - |
Rattus norvegicus | UDP + N-acetyl-D-galactosaminyl chondroitin oligosaccharide | - |
? | |
| UDP-N-acetyl-D-galactosamine + chondroitin oligosaccharide | best acceptor substrate | Bos taurus | UDP + N-acetyl-D-galactosaminyl chondroitin oligosaccharide | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Bos taurus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
- |
Bos taurus |
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