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Literature summary for 2.4.1.175 extracted from
- Molecular cloning and characterization of chondroitin polymerase from Escherichia coli strain K4 (2002), J. Biol. Chem., 277, 21567-21575.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| DNA and amino acid sequence determination and analysis, expression as soluble His-tagged protein in Escherichia coli strain TOP10 | Escherichia coli |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | complete inhibition | Escherichia coli |  |
| Cu2+ | complete inhibition | Escherichia coli | |
| EDTA | complete inhibition | Escherichia coli | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0316 | - |
UDP-N-acetyl-beta-D-galactosamine | - |
Escherichia coli | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | can partly substitute for Mn2+, 30.6% activity compared to Mn2+ | Escherichia coli | |
| Mg2+ | can partly substitute for Mn2+, 30.7% activity compared to Mn2+ | Escherichia coli | |
| Mn2+ | required, 20 mM | Escherichia coli | |
| Mn2+ | can partly be substituted by Fe2+ and Mg2+ | Escherichia coli | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-N-acetyl-alpha-D-galactosamine + chondroitin oligosaccharide | Escherichia coli | biosynthesis of K4 antigen, a capsule polysaccharide consisting of a chondroitin backbone to which beta-fructose is linked at position C-3 of the GlcNAc residue | UDP + N-acetyl-beta-D-galactosaminyl chondroitin oligosaccharide | - |
? | |
| UDP-N-acetyl-alpha-D-galactosamine + chondroitin sulfate oligosaccharide | Escherichia coli | - |
UDP + N-acetyl-beta-D-galactosaminyl chondroitin sulfate oligosaccharide | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | Q8L0V4 | - |
- |
| Escherichia coli K4 | Q8L0V4 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme expressed in E. coli strain TOP10 | Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.00053 | - |
purified rcombinant enzyme, substrate chondroitin polysaccharide with MW 10 kDa | Escherichia coli |
| 0.00124 | - |
purified rcombinant enzyme, substrate chondroitin hexasaccharide | Escherichia coli |
| 0.00331 | - |
purified rcombinant enzyme, substrate chondroitin sulfate polysaccharide with MW 20 kDa | Escherichia coli |
| 0.00334 | - |
purified rcombinant enzyme, substrate chondroitin sulfate hexasaccharide | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | the enzyme also has the 3-beta-glucuronosyltransferase, EC 2.4.1.226, activity | Escherichia coli | ? | - |
? | |
| additional information | the enzyme also has the 3-beta-glucuronosyltransferase, EC 2.4.1.226, activity | Escherichia coli K4 | ? | - |
? | |
| UDP-N-acetyl-alpha-D-galactosamine + (glucuronyl-N-acetyl-1,3-beta-D-galactosamine)2 | minimal required acceptor substrate chain length | Escherichia coli | UDP + N-acetyl-beta-D-galactosaminyl-1,4-glucuronyl-N-acetyl-1,3-beta-D-galactosaminyl-glucuronyl-N-acetyl-1,3-beta-D-galactosamine | - |
? | |
| UDP-N-acetyl-alpha-D-galactosamine + (glucuronyl-N-acetyl-1,3-beta-D-galactosamine)3 | - |
Escherichia coli | UDP + N-acetyl-beta-D-galactosaminyl-1,4-glucuronyl-N-acetyl-1,3-beta-D-galactosaminyl-(glucuronyl-N-acetyl-1,3-beta-D-galactosamine)2 | - |
? | |
| UDP-N-acetyl-alpha-D-galactosamine + (glucuronyl-N-acetyl-1,3-beta-D-galactosamine)3 | - |
Escherichia coli K4 | UDP + N-acetyl-beta-D-galactosaminyl-1,4-glucuronyl-N-acetyl-1,3-beta-D-galactosaminyl-(glucuronyl-N-acetyl-1,3-beta-D-galactosamine)2 | - |
? | |
| UDP-N-acetyl-alpha-D-galactosamine + chondroitin oligosaccharide | biosynthesis of K4 antigen, a capsule polysaccharide consisting of a chondroitin backbone to which beta-fructose is linked at position C-3 of the GlcNAc residue | Escherichia coli | UDP + N-acetyl-beta-D-galactosaminyl chondroitin oligosaccharide | - |
? | |
| UDP-N-acetyl-alpha-D-galactosamine + chondroitin sulfate oligosaccharide | - |
Escherichia coli | UDP + N-acetyl-beta-D-galactosaminyl chondroitin sulfate oligosaccharide | - |
? | |
| UDP-N-acetyl-beta-D-galactosamine + chondroitin sulfate hexasaccharide | - |
Escherichia coli | ? | - |
? | |
| UDP-N-acetyl-D-galactosamine + chondroitin oligosaccharide | acceptors are even-numbered chondroitin oligosaccharides with terminal glucuronyl-N-acetyl-1,3-beta-D-galactosamine sequence at non-reducing end, transfers N-acetyl-galactosamine to non-reducing glucuronic acid residues in beta-glycosidic linkage | Escherichia coli | UDP + N-acetyl-D-galactosaminyl chondroitin oligosaccharide | transfer in beta-1,4-linkage | ? | |
| UDP-N-acetyl-D-galactosamine + chondroitin oligosaccharide | acceptor substrates longer than a tetrasaccharide are absolutely required | Escherichia coli | UDP + N-acetyl-D-galactosaminyl chondroitin oligosaccharide | transfer in beta-1,4-linkage | ? | |
| UDP-N-acetyl-D-galactosamine + chondroitin oligosaccharide | acceptors are even-numbered chondroitin oligosaccharides with terminal glucuronyl-N-acetyl-1,3-beta-D-galactosamine sequence at non-reducing end, transfers N-acetyl-galactosamine to non-reducing glucuronic acid residues in beta-glycosidic linkage | Escherichia coli K4 | UDP + N-acetyl-D-galactosaminyl chondroitin oligosaccharide | transfer in beta-1,4-linkage | ? | |
| UDP-N-acetyl-D-galactosamine + chondroitin oligosaccharide | acceptor substrates longer than a tetrasaccharide are absolutely required | Escherichia coli K4 | UDP + N-acetyl-D-galactosaminyl chondroitin oligosaccharide | transfer in beta-1,4-linkage | ? | |
| UDP-N-acetyl-D-galactosamine + chondroitin sulfate oligosaccharide | acceptor substrates longer than a tetrasaccharide are absolutely required | Escherichia coli | UDP + N-acetyl-D-galactosaminyl chondroitin sulfate oligosaccharide | - |
? | |
| UDP-N-acetyl-D-galactosamine + chondroitin sulfate oligosaccharide | acceptor substrates longer than a tetrasaccharide are absolutely required | Escherichia coli K4 | UDP + N-acetyl-D-galactosaminyl chondroitin sulfate oligosaccharide | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| chondroitin polymerase | - |
Escherichia coli |
| More | the human form of this enzyme is a bifunctional glycosyltransferase, which also has the 3-beta-glucuronosyltransferase, EC 2.4.1.226, activity required for the synthesis of the chondroitin sulfate disaccharide repeats. Similar chondroitin synthase co-polymerases can be found in Pasteurella multocida and Escherichia coli. | Escherichia coli |
| More | the enzyme also has the 3-beta-glucuronosyltransferase, EC 2.4.1.226, activity | Escherichia coli |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
highest product chain length | Escherichia coli |
| 30 | - |
highest reaction rate | Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 7.5 | - |
Escherichia coli |
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