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Literature summary for 2.4.1.175 extracted from
- Molecular cloning and expression of human chondroitin N-acetylgalactosaminyltransferase. The key enzyme for chain initiation and elongation of chondroitin/dermatan sulfate on the protein linkage region tetrasaccharide shared by heparin/heparan sulfate (2002), J. Biol. Chem., 277, 8841-8846.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| DNA sequence determination, genomic organization and chromosome localization, functional expression as soluble protein fused to protein A IgG-binding domain in COS-1 cells, secretion into the medium | Homo sapiens |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | type II membrane protein topology | Homo sapiens | 16020 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mn2+ | - |
Homo sapiens |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 95000 | - |
x * 95000, recombinant enzyme, SDS-PAGE | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-N-acetyl-alpha-D-galactosamine + chondroitin oligosaccharide | Homo sapiens | involved in chondroitin biosynthesis | UDP + N-acetyl-beta-D-galactosaminyl chondroitin oligosaccharide | - |
? | |
| UDP-N-acetyl-alpha-D-galactosamine + chondroitin sulfate oligosaccharide | Homo sapiens | involved in chondroitin sulfate biosynthesis: elongation of growing chondroitin sulfate chain | UDP + N-acetyl-beta-D-galactosaminyl chondroitin sulfate oligosaccharide | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | Q8TDX6 | chondroitin N-acetylgalactosaminyltransferase I, not N-acetylgalactosaminyltransferase I EC 2.4.1.174 | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | 2 potential N-glycosylation sites, either one or both of which are used in COS-1 cells during expression of the recombinant fusion protein | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| bone marrow | - |
Homo sapiens | - |
| brain | - |
Homo sapiens | - |
| colon | low content | Homo sapiens | - |
| heart | - |
Homo sapiens | - |
| kidney | - |
Homo sapiens | - |
| leukocyte | peripheral blood | Homo sapiens | - |
| liver | - |
Homo sapiens | - |
| lung | - |
Homo sapiens | - |
| peripheral blood | - |
Homo sapiens | - |
| placenta | very high content | Homo sapiens | - |
| skeletal muscle | - |
Homo sapiens | - |
| small intestine | - |
Homo sapiens | - |
| spleen | - |
Homo sapiens | - |
| thymus | low content | Homo sapiens | - |
| thyroid gland | high content | Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | the enzyme also has N-acetylgalactosaminyltransferase I activity, EC 2.4.1.174 | Homo sapiens | ? | - |
? | |
| UDP-N-acetyl-alpha-D-galactosamine + chondroitin oligosaccharide | involved in chondroitin biosynthesis | Homo sapiens | UDP + N-acetyl-beta-D-galactosaminyl chondroitin oligosaccharide | - |
? | |
| UDP-N-acetyl-alpha-D-galactosamine + chondroitin sulfate oligosaccharide | involved in chondroitin sulfate biosynthesis: elongation of growing chondroitin sulfate chain | Homo sapiens | UDP + N-acetyl-beta-D-galactosaminyl chondroitin sulfate oligosaccharide | - |
? | |
| UDP-N-acetyl-D-galactosamine + chondroitin oligosaccharide | best acceptor substrate | Homo sapiens | UDP + N-acetyl-D-galactosaminyl chondroitin oligosaccharide | transfer in beta-1,4-linkage | ? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 95000, recombinant enzyme, SDS-PAGE | Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| chondroitin galactosaminyltransferase | - |
Homo sapiens |
| More | the enzyme also has N-acetylgalactosaminyltransferase I activity, EC 2.4.1.174 | Homo sapiens |
| More | the human form of this enzyme is a bifunctional glycosyltransferase, which also has the 3-beta-glucuronosyltransferase, EC 2.4.1.226, activity required for the synthesis of the chondroitin sulfate disaccharide repeats. Similar chondroitin synthase co-polymerases can be found in Pasteurella multocida and Escherichia coli. | Homo sapiens |
| More | the enzyme shows no 3-beta-glucuronosyltransferase activity, EC 2.4.1.226 | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
assay at | Homo sapiens |
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Release 2023.1 (January 2023)
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