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Literature summary for 2.4.1.175 extracted from
- Two N-acetylgalactosaminyltransferases are involved in the biosynthesis of chondroitin sulfate (1985), Eur. J. Biochem., 148, 463-469.
General Stability
| General Stability | Organism |
|---|---|
| ion-exchange chromatography on DEAE-trisacryl inactivates | Bos taurus |
| rapid thermodenaturation | Bos taurus |
| unstable upon purification | Bos taurus |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| microsome | - |
Bos taurus | - |
- |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-N-acetyl-alpha-D-galactosamine + chondroitin oligosaccharide | Bos taurus | involved in chondroitin biosynthesis | UDP + N-acetyl-beta-D-galactosaminyl chondroitin oligosaccharide | - |
? |  |
| UDP-N-acetyl-alpha-D-galactosamine + chondroitin sulfate oligosaccharide | Bos taurus | involved in chondroitin sulfate biosynthesis: elongation of growing chondroitin sulfate chain | UDP + N-acetyl-beta-D-galactosaminyl chondroitin sulfate oligosaccharide | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
calf | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partially solubilized with a buffer containing 2% Triton X-100, 20% glycerol and phospholipase A2 | Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| aorta thoracica | - |
Bos taurus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
34.6 cpm/min/mg protein | Bos taurus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-N-acetyl-alpha-D-galactosamine + (glucuronyl-N-acetyl-1,3-beta-D-galactosamine 4-sulfate)4 | i.e. chondroitin sulfate octasaccharide, glycosylated at 32% the rate of chondroitin octasaccharide | Bos taurus | UDP + N-acetyl-beta-D-galactosaminyl-1,4-glucuronyl-N-acetyl-1,3-beta-D-galactosaminyl-(glucuronyl-N-acetyl-1,3-beta-D-galactosamine)3 | - |
? | |
| UDP-N-acetyl-alpha-D-galactosamine + (glucuronyl-N-acetyl-1,3-beta-D-galactosamine)2 | i.e. chondroitin tetrasaccharide, glycosylated at 40% the rate of decamer glycosylation | Bos taurus | UDP + N-acetyl-beta-D-galactosaminyl-1,4-glucuronyl-N-acetyl-1,3-beta-D-galactosaminyl-glucuronyl-N-acetyl-1,3-beta-D-galactosamine | - |
? | |
| UDP-N-acetyl-alpha-D-galactosamine + (glucuronyl-N-acetyl-1,3-beta-D-galactosamine)3 | i.e. chondroitin hexasaccharide, glycosylated at 60% the rate of decamer glycosylation | Bos taurus | UDP + N-acetyl-beta-D-galactosaminyl-1,4-glucuronyl-N-acetyl-1,3-beta-D-galactosaminyl-(glucuronyl-N-acetyl-1,3-beta-D-galactosamine)2 | - |
? | |
| UDP-N-acetyl-alpha-D-galactosamine + (glucuronyl-N-acetyl-1,3-beta-D-galactosamine)4 | i.e. chondroitin octasaccharide, glycosylated at 75% the rate of decamer glycosylation | Bos taurus | UDP + N-acetyl-beta-D-galactosaminyl-1,4-glucuronyl-N-acetyl-1,3-beta-D-galactosaminyl-(glucuronyl-N-acetyl-1,3-beta-D-galactosamine)3 | - |
? | |
| UDP-N-acetyl-alpha-D-galactosamine + (glucuronyl-N-acetyl-1,3-beta-D-galactosamine)5 | i.e. chondroitin decasaccharide, best substrate | Bos taurus | UDP + N-acetyl-beta-D-galactosaminyl-1,4-glucuronyl-N-acetyl-1,3-beta-D-galactosaminyl-(glucuronyl-N-acetyl-1,3-beta-D-galactosamine)4 | - |
? | |
| UDP-N-acetyl-alpha-D-galactosamine + (glucuronyl-N-acetyl-1,3-beta-D-galactosamine)6 | i.e. chondroitin dodecasaccharide, glycosylated at about 60% the rate of decamer glycosylation | Bos taurus | UDP + N-acetyl-beta-D-galactosaminyl-1,4-glucuronyl-N-acetyl-1,3-beta-D-galactosaminyl-(glucuronyl-N-acetyl-1,3-beta-D-galactosamine)5 | - |
? | |
| UDP-N-acetyl-alpha-D-galactosamine + (glucuronyl-N-acetyl-1,3-beta-D-galactosamine)7 | i.e. chondroitin tetradecasaccharide, glycosylated at about 45% the rate of decamer glycosylation | Bos taurus | UDP + N-acetyl-beta-D-galactosaminyl-1,4-glucuronyl-N-acetyl-1,3-beta-D-galactosaminyl-(glucuronyl-N-acetyl-1,3-beta-D-galactosamine)6 | - |
? | |
| UDP-N-acetyl-alpha-D-galactosamine + (glucuronyl-N-acetyl-1,3-beta-D-galactosamine)8 | i.e. chondroitin hexadecasaccharide, glycosylated at 15% the rate of decamer glycosylation | Bos taurus | UDP + N-acetyl-beta-D-galactosaminyl-1,4-glucuronyl-N-acetyl-1,3-beta-D-galactosaminyl-(glucuronyl-N-acetyl-1,3-beta-D-galactosamine)7 | - |
? | |
| UDP-N-acetyl-alpha-D-galactosamine + (glucuronyl-N-acetyl-1,3-beta-D-glucosamine)4 | i.e. hyaluronate octasaccharide, glycosylated at 25% the rate of chondroitin octasaccharide | Bos taurus | UDP + ? | - |
? | |
| UDP-N-acetyl-alpha-D-galactosamine + chondroitin oligosaccharide | involved in chondroitin biosynthesis | Bos taurus | UDP + N-acetyl-beta-D-galactosaminyl chondroitin oligosaccharide | - |
? | |
| UDP-N-acetyl-alpha-D-galactosamine + chondroitin sulfate oligosaccharide | involved in chondroitin sulfate biosynthesis: elongation of growing chondroitin sulfate chain | Bos taurus | UDP + N-acetyl-beta-D-galactosaminyl chondroitin sulfate oligosaccharide | - |
? | |
| UDP-N-acetyl-D-galactosamine + chondroitin oligosaccharide | no substrates are glucuronyl-N-acetyl-1,3-beta-galactosamine or glucuronyl-N-acetyl-1,3-beta-D-galactosaminyl-N-acetyl-1,3-beta-D-galactosaminyl-N-acetyl-galactosaminol | Bos taurus | UDP + N-acetyl-D-galactosaminyl chondroitin oligosaccharide | - |
? | |
| UDP-N-acetyl-D-galactosamine + chondroitin oligosaccharide | acceptors are even-numbered chondroitin oligosaccharides with terminal glucuronyl-N-acetyl-1,3-beta-D-galactosamine sequence at non-reducing end, transfers N-acetyl-galactosamine to non-reducing glucuronic acid residues in beta-glycosidic linkage | Bos taurus | UDP + N-acetyl-D-galactosaminyl chondroitin oligosaccharide | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Bos taurus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
t1/2: 5 min, 90% loss of activity within 20 min | Bos taurus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
- |
Bos taurus |
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Release 2023.1 (January 2023)
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