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Literature summary for 2.4.1.174 extracted from

  • Rohrmann, K.; Niemann, R.; Buddecke, E.
    Two N-acetylgalactosaminyltransferases are involved in the biosynthesis of chondroitin sulfate (1985), Eur. J. Biochem., 148, 463-469.
    View publication on PubMed
Search for more literature for 2.4.1.174

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
 Bos taurus
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ requirement Bos taurus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-N-acetyl-D-galactosamine + glucuronylgalactosyl glycosides Bos taurus involved in chondroitin sulfate biosynthesis: transfer of the first N-acetylgalactosamine residue UDP + N-acetyl-D-galactosaminylglucuronylgalactosyl glycosides
-
 ?
UDP-N-acetyl-D-galactosamine + glucuronylgalactosyl glycosides Bos taurus involved in the biosynthesis of chodroitin sulfate. Key enzyme activity for the initiation of chondroitin and dermatan sulfates, transferring GalNAc to the GlcA-Gal-Gal-Xyl-Ser core. UDP + N-acetyl-D-galactosaminylglucuronylgalactosyl glycosides
-
 ?

Organism

Organism UniProt Comment Textmining
Bos taurus
-
 calf
-

Purification (Commentary)

Purification (Comment) Organism
partially solubilized with a buffer containing 2% Triton X-100, 20% glycerol and phospholipase A2 Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
aorta thoracica
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 13.1 cpm/min/mg protein Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-N-acetyl-D-galactosamine + beta-D-glucuronyl-1,3-beta-D-galactose minimum requirement for acceptor structure Bos taurus UDP + N-acetyl-D-galactosamine-1,4-beta-D-glucuronyl-1,3-beta-D-galactose
-
 ?
UDP-N-acetyl-D-galactosamine + beta-D-glucuronyl-1,3-beta-D-galactosyl-1,3-beta-D-galactose best substrate Bos taurus UDP + N-acetyl-D-galactosaminyl-1,4-beta-D-glucuronyl-1,3-beta-D-galactosyl-1,3-beta-D-galactose
-
 ?
UDP-N-acetyl-D-galactosamine + beta-D-glucuronyl-1,3-beta-D-galactosyl-1,4-beta-D-glucose
-
 Bos taurus UDP + N-acetyl-D-galactosamine-1,4-beta-D-glucuronyl-1,3-beta-D-galactosyl-1,4-beta-D-glucose
-
 ?
UDP-N-acetyl-D-galactosamine + glucuronylgalactosyl glycosides acceptors are tri- or disaccharides with terminal glucuronyl residue and a galactosyl residue in penultimate position, transfers acetylgalactosamine in beta-linkage, no substrates are glucuronic acid or disaccharides with terminal galactosyl residues Bos taurus UDP + N-acetyl-D-galactosaminyl-1,4-beta-D-glucuronylgalactosyl glycosides
-
 ?
UDP-N-acetyl-D-galactosamine + glucuronylgalactosyl glycosides involved in chondroitin sulfate biosynthesis: transfer of the first N-acetylgalactosamine residue Bos taurus UDP + N-acetyl-D-galactosaminylglucuronylgalactosyl glycosides
-
 ?
UDP-N-acetyl-D-galactosamine + glucuronylgalactosyl glycosides involved in the biosynthesis of chodroitin sulfate. Key enzyme activity for the initiation of chondroitin and dermatan sulfates, transferring GalNAc to the GlcA-Gal-Gal-Xyl-Ser core. Bos taurus UDP + N-acetyl-D-galactosaminylglucuronylgalactosyl glycosides
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Bos taurus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 1 h, 10% loss of activity Bos taurus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.4
-
-
 Bos taurus