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Literature summary for 2.4.1.17 extracted from

  • Zhang, H.; Patana, A.S.; Mackenzie, P.I.; Ikushiro, S.; Goldman, A.; Finel, M.
    Human UDP-glucuronosyltransferase expression in insect cells: ratio of active to inactive recombinant proteins and the effects of a C-terminal his-tag on glucuronidation kinetics (2012), Drug Metab. Dispos., 40, 1935-1944.
    View publication on PubMed

Application

Application Comment Organism
analysis after infection of Sf9 insect cells with increasing amounts of recombinant baculovirus, encoding either UGT1A9 or UGT2B7, the correlation between glucuronidation rates and degree of infection follows different trends, depending on whether activity is the actual activity measured or is corrected for UDP-glucuronosyltransferase expression level. Above a certain low level of infection, further increases in infection ratios lead to a large decline in normalized activity, presumably due to the presence of full-length but inactive enzyme in the sample. Inaccuracies in comparison of normalized activity between different batches of a recombinant UDP-glucuronosyltransferase can be reduced by lowering the degree of infection of the insect cells, in combination with careful monitoring of UDP-glucuronosyltransferase expression. Poly-His-containing peptides, fused to the UDP-glucuronosyltransferase C-terminus, allow sensitive immunodetection of expressed enzymes with monoclonal antibodies. A minor increase in the Km values has been detected in the His-tagged UDP-glucuronosyltransferases, but no changes in parameters such as the kinetic model and the effects of albumin addition Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
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