Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant isozymes as C-terminal Histagged fusion proteins in baculovirus-infected insect cells, expression levels, overview | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
H369A | site-directed mutagenesis of isozyme UGT1A9, the Km values for scopoletin and UDP-D-glucuronate are increased compared to the wild-type isozyme, the mutant shows impaired cosubstrate binding | Homo sapiens |
H371A | site-directed mutagenesis of isozyme UGT1A6, the Km values for scopoletin and UDP-D-glucuronate are increased by 4 and 11fold, respectively, compared to the wild-type isozyme, the mutant shows severely impaired cosubstrate binding | Homo sapiens |
H377A | site-directed mutagenesis of isozyme UGT1A9, inactive mutant | Homo sapiens |
H379A | site-directed mutagenesis of isozyme UGT1A6, the Km values for scopoletin and UDP-D-glucuronate are increased compared to the wild-type isozyme, the mutant shows severely impaired cosubstrate binding | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | bisubstrate kinetics of wild-type and mutant isozyme UGT1A6 | Homo sapiens | |
0.048 | - |
1-naphthol | H379A mutant isozyme UGT1A6 | Homo sapiens | |
0.08 | - |
scopoletin | wild-type isozyme UGT1A9 | Homo sapiens | |
0.083 | - |
1-naphthol | H371A mutant isozyme UGT1A6 | Homo sapiens | |
0.17 | - |
4-Methylumbelliferone | wild-type isozyme UGT1A6 | Homo sapiens | |
0.29 | - |
scopoletin | wild-type isozyme UGT1A6 | Homo sapiens | |
0.58 | - |
4-Methylumbelliferone | H379A mutant isozyme UGT1A6 | Homo sapiens | |
0.66 | - |
4-Methylumbelliferone | H371A mutant isozyme UGT1A6 | Homo sapiens | |
1.64 | - |
scopoletin | H369A mutant isozyme UGT1A9 | Homo sapiens | |
3.88 | - |
scopoletin | H371A mutant isozyme UGT1A6 | Homo sapiens | |
7.64 | - |
scopoletin | H379A mutant isozyme UGT1A6 | Homo sapiens | |
27 | - |
1-naphthol | wild-type isozyme UGT1A6 | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
endoplasmic reticulum membrane | - |
Homo sapiens | 5789 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-D-glucuronate + acceptor | Homo sapiens | UDP-glucuronosyltransferases play important roles in the metabolism, detoxification,and clearance of many different xenobiotics, including drugs and endogenous compounds | UDP + acceptor beta-D-glucuronoside | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
isozyme UGT1A6 | - |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
UDP-alpha-D-glucuronate + acceptor = UDP + acceptor beta-D-glucuronoside | compulsory ordered bi bi mechanism | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-D-glucuronate + 1-naphthol | - |
Homo sapiens | UDP + 1-naphthyl beta-D-glucuronoside | - |
? | |
UDP-D-glucuronate + 4-methylumbelliferone | - |
Homo sapiens | UDP + 4-methylumbelliferyl beta-D-glucuronoside | - |
? | |
UDP-D-glucuronate + acceptor | - |
Homo sapiens | UDP + acceptor beta-D-glucuronoside | - |
? | |
UDP-D-glucuronate + acceptor | UDP-glucuronosyltransferases play important roles in the metabolism, detoxification,and clearance of many different xenobiotics, including drugs and endogenous compounds | Homo sapiens | UDP + acceptor beta-D-glucuronoside | - |
? | |
UDP-D-glucuronate + scopoletin | substrate binding structure, UGT1A6 residues His371 and Glu379 are directly involved in UDPGA binding but are not the general acid or general base, homology mocelling, overview | Homo sapiens | UDP + scopoletin beta-D-glucuronoside | - |
? | |
UDP-D-glucuronate + umbelliferone | - |
Homo sapiens | UDP + umbelliferyl beta-D-glucuronoside | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | substrate binding structure, homology mnodelling of wild-type and mutant isozymes UGT1A6 and UGT1A9, overview | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
UDP-glucuronosyltransferase | - |
Homo sapiens |
UGT | - |
Homo sapiens |