Cloned (Comment) | Organism |
---|---|
expression of UGT1A10 in baculovirus-infected Sf9 insect cells as His-tagged protein | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
F90A | exhibits little or no activity towards estrone, estradiol and estriol. Complete loss of activity with 2-hydroxyestrone, 2-hydroxyestradiol, 4-hydroxyestradiol and 4-hydroxyestrone | Homo sapiens |
F90L | 86% decrease in activity towards estrone, increase in affinity. Decrease in catalytic activity toward estradiol of 64%, and an almost 3fold increase in affinity. Estriol is not glucuronated by the mutant enzyme. 72% increase in activity towards 2-hydroxyestrone. 78% decrease in activity towards 4-hydroxyestrone. Increase in activity towards 4-hydroxyestradiol | Homo sapiens |
F93A | 75% decrease in activity towards estrone. For estradiol, the F93A mutant showes a slight impairment of activity with 30% decrease in Vmax. The Km for estradiol decreases approximately 2fold. 59% decrease in activity towards estriol, similar KM-value. 99% decrease in activity towards 2-hydroxyestrone. 69% decrease in activity towards 4-hydroxyestrone, increase in affinity. 93% decrease in activity topwards 4-hydroxyestradiol, increase in affinity | Homo sapiens |
F93L | exhibits little or no activity towards estrone, estradiol and estriol. 88% decrease in activity towards 2-hydroxyestrone, increase in affinity. Complete loss in activity towards 4-hydroxyestrone and 4-hydroxyestradiol | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0049 | - |
estradiol | 37°C, pH 8.0, mutant enzyme F90L | Homo sapiens | |
0.0063 | - |
estrone | 37°C, pH 8.0, mutant enzyme F93A | Homo sapiens | |
0.0078 | - |
estradiol | 37°C, pH 8.0, mutant enzyme F93A | Homo sapiens | |
0.0102 | - |
2-hydroxyestrone | 37°C, pH 8.0, mutant enzyme F93A | Homo sapiens | |
0.0138 | - |
estradiol | 37°C, pH 8.0, wild-type enzyme | Homo sapiens | |
0.0388 | - |
estriol | 37°C, pH 8.0, wild-type enzyme | Homo sapiens | |
0.0401 | - |
4-hydroxyestradiol | 37°C, pH 8.0, mutant enzyme F93A | Homo sapiens | |
0.0401 | - |
4-hydroxyestrone | 37°C, pH 8.0, mutant enzyme F93A | Homo sapiens | |
0.0421 | - |
estrone | 37°C, pH 8.0, wild-type enzyme | Homo sapiens | |
0.0467 | - |
estriol | 37°C, pH 8.0, mutant enzyme F93A | Homo sapiens | |
0.0823 | - |
2-hydroxyestrone | 37°C, pH 8.0, mutant enzyme F93L | Homo sapiens | |
0.1182 | - |
4-hydroxyestrone | 37°C, pH 8.0, wild-type enzyme | Homo sapiens | |
0.1305 | - |
2-hydroxyestrone | 37°C, pH 8.0, wild-type enzyme | Homo sapiens | |
0.2338 | - |
4-hydroxyestradiol | 37°C, pH 8.0, wild-type enzyme | Homo sapiens | |
0.291 | - |
2-hydroxyestrone | 37°C, pH 8.0, mutant enzyme F90L | Homo sapiens | |
0.292 | - |
4-hydroxyestradiol | 37°C, pH 8.0, mutant enzyme F90L | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microsome | - |
Homo sapiens | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
estrogen + UDP-glucuronate | Homo sapiens | UDP-glucuronosyltransferase 1A10 has been identified as the major isoform involved in the biotransformation of a wide range of phenolic substrates, including native estrogens and their oxidized metabolites | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q9HAW8 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-hydroxyestradiol + UDP-glucuronate | - |
Homo sapiens | ? | - |
? | |
2-hydroxyestrone + UDP-glucuronate | - |
Homo sapiens | ? | - |
? | |
4-hydroxyestradiol + UDP-glucuronate | - |
Homo sapiens | ? | - |
? | |
4-hydroxyestrone + UDP-glucuronate | - |
Homo sapiens | ? | - |
? | |
estradiol + UDP-glucuronate | - |
Homo sapiens | ? | - |
? | |
estriol + UDP-glucuronate | - |
Homo sapiens | ? | - |
? | |
estrogen + UDP-glucuronate | UDP-glucuronosyltransferase 1A10 has been identified as the major isoform involved in the biotransformation of a wide range of phenolic substrates, including native estrogens and their oxidized metabolites | Homo sapiens | ? | - |
? | |
estrogen + UDP-glucuronate | phenylalanine(90) and phenylalanine(93) are crucial amino acids within the estrogen binding site of the human UDP-glucuronosyltransferase 1A10 | Homo sapiens | ? | - |
? | |
estrone + UDP-glucuronate | - |
Homo sapiens | UDP + estrone 3-O-glucuronide | - |
? |
Synonyms | Comment | Organism |
---|---|---|
UDP-glucuronosyltransferase 1A10 | - |
Homo sapiens |