Activating Compound | Comment | Organism | Structure |
---|---|---|---|
phosphatidylinositol | highly required for activity of GlcAT-P and GlcAT-S with paraglobosid and other glycolipids | Homo sapiens | |
sphingomyelin | almost indispensable activator of GlcAT-P as to glycoprotein acceptors | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
functional expression of FLAG-tagged enzymes GlcAT-P and GlcAT-S in Escherichia coli strain BL21 as soluble proteins | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
asialo-orosomucoid | inhibits activity with paragloboside | Homo sapiens | |
lacto-N-biose | 22% inhibition of recombinant GlcAT-P and 77.3% of recombinant GlcAT-S at 5 mM | Homo sapiens | |
lactose | 24.5% inhibition of recombinant GlcAT-S at 5 mM, no inhibition of recombinant GlcAT-P | Homo sapiens | |
N-acetyllactosamine | 87.4% inhibition of recombinant GlcAT-P and 83.7% of recombinant GlcAT-S at 5 mM | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
32000 | - |
x * 32000, recombinant GlcAT-P and GlcAT-S, SDS-PAGE | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | enzyme are involved in biosynthesis of HNK-1 carbohydrate | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
soluble recombinant FLAG-tagged enzymes GlcAT-P and GlcAT-S, 1233fold and 7511fold, respectively, from Escherichia coli by affinity chromatographic steps | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
nervous system | - |
Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.45 | - |
purified recombinant GlcAT-S with substrate asialo-orosomucoid | Homo sapiens |
1.11 | - |
purified recombinant GlcAT-P with substrate asialo-orosomucoid | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | enzyme are involved in biosynthesis of HNK-1 carbohydrate | Homo sapiens | ? | - |
? | |
additional information | the recombinant GlcAT-P is highly specific for terminal type II structure, Galbeta1-4GlcNAc, while the recombinant GlcAT-S recognizes type I, Galbeta1-3GlcNAc, and type II structures | Homo sapiens | ? | - |
? | |
UDP-glucuronate + asialo-orosomucoid | acceptor is a glycoprotein, preferred substrate | Homo sapiens | UDP + asialo-orosomucoid beta-D-glucuronide | - |
? | |
UDP-glucuronate + paragloboside | acceptor is a glycolipid, activity requires phosphatidylinositol | Homo sapiens | UDP + paragloboside beta-D-glucuronide | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 32000, recombinant GlcAT-P and GlcAT-S, SDS-PAGE | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
GlcAT-P | - |
Homo sapiens |
GlcAT-S | - |
Homo sapiens |
glucuronyltransferase | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
assay at | Homo sapiens |