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Literature summary for 2.4.1.17 extracted from
- An active and water-soluble truncation mutant of the human UDP-glucuronosyltransferase 1A9 (2004), Mol. Pharmacol., 65, 826-831.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of isozyme UGT1A9 lacking 45 residues of the C-terminus including the trans-membrane helix, enzyme mutant is His6-tagged at the shortened C-terminus, detergent-free expression as soluble protein in insect cells | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | either detergent binding to the transmembrane helix within the full-length isozyme UGT1A9 or removal of this helix affect the interaction of the enzyme with its aglycone substrate in a similar manner | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Triton X-100 | inhibition of recombinant soluble isozyme UGT1A9 | Homo sapiens |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetics | Homo sapiens |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | integral | Homo sapiens | 16020 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Homo sapiens | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | O60656 | isozyme UGT1A9; isozyme UGT1A9 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged isozyme UGT1A9 mutant from insect cells by nickel affinity chromatogaphy | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | substrate specificities of wild-type and recombinant soluble mutant isozyme UGT1A9 | Homo sapiens | ? | - |
? | |
| UDP-glucuronate + 1-naphthol | - |
Homo sapiens | UDP + 1-naphthol beta-D-glucuronide | - |
? | |
| UDP-glucuronate + 4-nitrophenol | - |
Homo sapiens | UDP + 4-nitrophenol beta-D-glucuronide | - |
? | |
| UDP-glucuronate + entacapone | - |
Homo sapiens | UDP + entacapone beta-D-glucuronide | - |
? | |
| UDP-glucuronate + scopoletin | - |
Homo sapiens | UDP + scopoletin beta-D-glucuronide | - |
? | |
| UDP-glucuronate + umbelliferone | - |
Homo sapiens | UDP + umbelliferone beta-D-glucuronide | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | - |
Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| UDP-glucuronosyltransferase | - |
Homo sapiens |
| UGT | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Homo sapiens |
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Release 2023.1 (January 2023)
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