Cloned (Comment) | Organism |
---|---|
stable expression of isozymes UGT1A1, UGT1A3, UGT1A6, UGT1A8, UGT1A9, UGT1A10, and UGT2B7 in HEK 293 cells, isozymes UGT2B4, UGT2B7, UGT2B10, and UGT2B15 are inactivated during cell lysis for microsome preparation | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics | Homo sapiens | |
0.42 | - |
morphine | morphine 3-glucuronide formation, recombinant isozyme UGT2B7, pH 7.4, 37°C | Homo sapiens | |
0.97 | - |
morphine | morphine 6-glucuronide formation, recombinant isozyme UGT2B7, pH 7.4, 37°C | Homo sapiens | |
2.6 | - |
morphine | morphine 3-glucuronide formation, recombinant isozyme UGT1A8, pH 7.4, 37°C | Homo sapiens | |
3.2 | - |
morphine | morphine 3-glucuronide formation, recombinant isozyme UGT1A3, pH 7.4, 37°C | Homo sapiens | |
12.6 | - |
morphine | morphine 3-glucuronide formation, recombinant isozyme UGT1A10, pH 7.4, 37°C | Homo sapiens | |
18 | - |
morphine | morphine 3-glucuronide formation, recombinant isozyme UGT1A6, pH 7.4, 37°C | Homo sapiens | |
18.7 | - |
morphine | morphine 3-glucuronide formation, recombinant isozyme UGT1A1, pH 7.4, 37°C | Homo sapiens | |
37.4 | - |
morphine | morphine 3-glucuronide formation, recombinant isozyme UGT1A9, pH 7.4, 37°C | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Homo sapiens | 16020 | - |
microsome | - |
Homo sapiens | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | isozyme UGT2B7 is the major isozyme involved in metabolization of morphine in the liver | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
several enzyme isoforms | - |
Purification (Comment) | Organism |
---|---|
microsome preparation of HEK 293 cells expressing recombinant isozymes | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
gastrointestinal tract | isozymes UGT1A1, UGT1A3, UGT1A6, UGT1A8, UGT1A9, UGT1A10, and UGT2B7 | Homo sapiens | - |
liver | isozymes UGT1A1, UGT1A3, UGT1A6, UGT1A9, and UGT2B7 | Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | isozyme UGT2B7 is the major isozyme involved in metabolization of morphine in the liver | Homo sapiens | ? | - |
? | |
UDP-glucuronate + 4-methylumbelliferone | all isozymes, nonselective substrate | Homo sapiens | UDP + 4-methylumbelliferyl-beta-D-glucuronide | - |
? | |
UDP-glucuronate + morphine | isozymes UGT1A1, UGT1A3, UGT1A6, UGT1A8, UGT1A9, UGT1A10, and UGT2B7 | Homo sapiens | UDP + morphine-3-beta-D-glucuronide | - |
? | |
UDP-glucuronate + morphine | isozyme UGT2B7 | Homo sapiens | UDP + morphine-6-beta-D-glucuronide | - |
? |
Synonyms | Comment | Organism |
---|---|---|
More | enzymes belongs to the 2B subfamily | Homo sapiens |
UDP-glucuronosyltransferase | - |
Homo sapiens |
UGT | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Homo sapiens |