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Literature summary for 2.4.1.17 extracted from

  • Kurkela, M.; Garcia-Horsman, J.A.; Luukkanen, L.; Moersky, S.; Taskinen, J.; Baumann, M.; Kostiainen, R.; Hirvonen, J.; Finel, M.
    Expression and Characterization of Recombinant Human UDP-glucuronosyltransferases (UGTs) (2003), J. Biol. Chem., 278, 3536-3544.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
Phospholipids
-
Homo sapiens
Triton X-100 stimulates UGT1A9 activity Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
8 different UGT's expressed in baculovirus-infected cells as His-tagged proteins Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
Triton X-100 inhibition of UGT2B and almost all of the UGT1A isozymes, not inhibit glucuronidation of 1-naphthol nor scopoletin by UGT1A9, inhibition of entacapone glucuronidation tested on isozymes of UGT1A and UGT1B families, only for UGT1A9 showed reversible inhibition Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.061
-
scopoletin apparent Km for membrane-bound UGT1AB Homo sapiens
0.229
-
UDP-glucuronic acid apparent Km for membrane-bound UGT1AB Homo sapiens
0.704
-
UDP-glucuronic acid apparent Km for purified UGT1AB Homo sapiens
0.79
-
scopoletin apparent Km for purified UGT1AB Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
endoplasmic reticulum
-
Homo sapiens 5783
-
microsome
-
Homo sapiens
-
-
nuclear membrane
-
Homo sapiens 31965
-

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
His-tagged UGT1A9 purified by immobilized metal-chelating chromatography Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-naphthol + UDP-glucuronate
-
Homo sapiens naphth-1-yl-beta-D-glucuronide + UDP
-
?
scopoletin + UDPglucuronate
-
Homo sapiens UDP + scopoletin O-glucuronide
-
?

Subunits

Subunits Comment Organism
? suggested homodimer Homo sapiens
? all values determined by SDS-PAGE Homo sapiens