Activating Compound | Comment | Organism | Structure |
---|---|---|---|
cysteine | 10 mM, slight activation | Sus scrofa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ATP | inhibitor at 10 mM, no effect below | Sus scrofa | |
diclofenac | - |
Sus scrofa | |
estrone | competitive to estradiol-17beta | Sus scrofa | |
UDP | 0.05 mM inhibits 60% of the activity of the mitochondrial enzyme | Sus scrofa | |
Zn2+ | inhibitor above 10mM | Sus scrofa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.045 | - |
17beta-estradiol | - |
Sus scrofa | |
0.046 | - |
estradiol-17beta | range between 0.045 mM and 0.047 mM | Sus scrofa | |
0.16 | - |
UDPglucuronic acid | - |
Sus scrofa |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Sus scrofa | 5829 | - |
microsome | - |
Sus scrofa | - |
- |
mitochondrion | - |
Sus scrofa | 5739 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 20 mM, activation | Sus scrofa | |
Mg2+ | activation at 20 mM | Sus scrofa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sus scrofa | - |
pig | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
small intestine | - |
Sus scrofa | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
estradiol-17beta + UDPglucuronate | - |
Sus scrofa | estradiol-17beta-3-monoglucuronide + UDP | - |
? | |
estriol + UDPglucuronate | - |
Sus scrofa | UDP + estriol-16 alpha-glucuronide | - |
? | |
estrone + UDPglucuronate | - |
Sus scrofa | UDP + estrone 3-O-glucuronide | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
42 | - |
activity increases with rise in incubation temperature | Sus scrofa |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
inactivation in absence of substrates | Sus scrofa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
Tris/HCl buffer, in Tris/maleate and phosphate buffers the enzyme shows this pH optimum but lower activity | Sus scrofa |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.008 | - |
diclofenac | - |
Sus scrofa |