Cloned (Comment) | Organism |
---|---|
gene CHS1, expression of YFP-tagged wild-type and mutant isozymes in strain BWP17, location of the YFP-tagged Chs proteins in yeast and hyphal cells, overview | Candida albicans |
gene CHS2, expression of YFP-tagged wild-type and mutant isozymes in strain BWP17, location of the YFP-tagged Chs proteins in yeast and hyphal cells, overview | Candida albicans |
gene CHS3, expression of YFP-tagged wild-type and mutant isozymes in strain BWP17, location of the YFP-tagged Chs proteins in yeast and hyphal cells, overview | Candida albicans |
gene CHS8, expression of YFP-tagged wild-type and mutant isozymes in strain BWP17, location of the YFP-tagged Chs proteins in yeast and hyphal cells, overview | Candida albicans |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of isozyme deficient mutant cells, analysis of chitin ghosts of chs mutant strains by shadow-cast transmission electron microscopy show that the long-chitin microfibrils are absent in chs8 mutants and the short-chitin rodlets are absent in chs3 mutants. The inferred site of chitin microfibril synthesis of these Chs enzymes is corroborated by their localization determined in Chsp-YFP-expressing strains, overview | Candida albicans |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cell wall | - |
Candida albicans | 5618 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Candida albicans | shape and integrity of fungal cells is dependent on the skeletal polysaccharides in their cell walls of which beta-1,3-glucan and chitin are of principle importance, individual chitin synthase enzymes synthesize microfibrils of differing structure at specific locations in the Candida albicans cell wall, overview | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Candida albicans | A0A1D8PFK5 | Chs8; isozyme Chs8, gene CHS8 | - |
Candida albicans | P23316 | Chs1; isozyme Chs1, gene CHS1 | - |
Candida albicans | P30572 | Chs2; isozymeChs2, gene CHS2 | - |
Candida albicans | P30573 | Chs3; isozyme Chs3, gene CHS3 | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
hypha | - |
Candida albicans | - |
additional information | the architecture of the chitin skeleton of Candida albicans is shaped by the action of more than one chitin synthase at the site of cell wall synthesis | Candida albicans | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
recombinant YFP-tagged isozymes in BWP17 cells | Candida albicans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | shape and integrity of fungal cells is dependent on the skeletal polysaccharides in their cell walls of which beta-1,3-glucan and chitin are of principle importance, individual chitin synthase enzymes synthesize microfibrils of differing structure at specific locations in the Candida albicans cell wall, overview | Candida albicans | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Chs1 | - |
Candida albicans |
Chs2 | - |
Candida albicans |
CHS3 | - |
Candida albicans |
Chs8 | - |
Candida albicans |