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Literature summary for 2.4.1.16 extracted from

  • Lenardon, M.D.; Whitton, R.K.; Munro, C.A.; Marshall, D.; Gow, N.A.
    Individual chitin synthase enzymes synthesize microfibrils of differing structure at specific locations in the Candida albicans cell wall (2007), Mol. Microbiol., 66, 1164-1173.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene CHS1, expression of YFP-tagged wild-type and mutant isozymes in strain BWP17, location of the YFP-tagged Chs proteins in yeast and hyphal cells, overview Candida albicans
gene CHS2, expression of YFP-tagged wild-type and mutant isozymes in strain BWP17, location of the YFP-tagged Chs proteins in yeast and hyphal cells, overview Candida albicans
gene CHS3, expression of YFP-tagged wild-type and mutant isozymes in strain BWP17, location of the YFP-tagged Chs proteins in yeast and hyphal cells, overview Candida albicans
gene CHS8, expression of YFP-tagged wild-type and mutant isozymes in strain BWP17, location of the YFP-tagged Chs proteins in yeast and hyphal cells, overview Candida albicans

Protein Variants

Protein Variants Comment Organism
additional information construction of isozyme deficient mutant cells, analysis of chitin ghosts of chs mutant strains by shadow-cast transmission electron microscopy show that the long-chitin microfibrils are absent in chs8 mutants and the short-chitin rodlets are absent in chs3 mutants. The inferred site of chitin microfibril synthesis of these Chs enzymes is corroborated by their localization determined in Chsp-YFP-expressing strains, overview Candida albicans

Localization

Localization Comment Organism GeneOntology No. Textmining
cell wall
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Candida albicans 5618
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Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Candida albicans shape and integrity of fungal cells is dependent on the skeletal polysaccharides in their cell walls of which beta-1,3-glucan and chitin are of principle importance, individual chitin synthase enzymes synthesize microfibrils of differing structure at specific locations in the Candida albicans cell wall, overview ?
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?

Organism

Organism UniProt Comment Textmining
Candida albicans A0A1D8PFK5 Chs8; isozyme Chs8, gene CHS8
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Candida albicans P23316 Chs1; isozyme Chs1, gene CHS1
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Candida albicans P30572 Chs2; isozymeChs2, gene CHS2
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Candida albicans P30573 Chs3; isozyme Chs3, gene CHS3
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Source Tissue

Source Tissue Comment Organism Textmining
hypha
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Candida albicans
-
additional information the architecture of the chitin skeleton of Candida albicans is shaped by the action of more than one chitin synthase at the site of cell wall synthesis Candida albicans
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Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
recombinant YFP-tagged isozymes in BWP17 cells Candida albicans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information shape and integrity of fungal cells is dependent on the skeletal polysaccharides in their cell walls of which beta-1,3-glucan and chitin are of principle importance, individual chitin synthase enzymes synthesize microfibrils of differing structure at specific locations in the Candida albicans cell wall, overview Candida albicans ?
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?

Synonyms

Synonyms Comment Organism
Chs1
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Candida albicans
Chs2
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Candida albicans
CHS3
-
Candida albicans
Chs8
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Candida albicans