Literature summary for 2.4.1.16 extracted from

Kang, T.H.; Hwang, E.I.; Yun, B.S.; Shin, C.S.; Kim, S.U.
Chitin synthase 2 inhibitory activity of O-methyl pisiferic acid and 8,20-dihydroxy-9(11),13-abietadien-12-one, isolated from Chamaecyparis pisifera (2008), Biol. Pharm. Bull., 31, 755-759.

Search for more literature for 2.4.1.16

Inhibitors

Inhibitors	Comment	Organism	Structure
8,20-dihydroxy-9(11),13-abietadien-12-one	-	Candida albicans
8,20-dihydroxy-9(11),13-abietadien-12-one	a diterpene compoud isolated from leaves of Chamaecyparis pisifera, inhibits Chs1p; a diterpene compoud isolated from leaves of Chamaecyparis pisifera, inhibits Chs2p	Saccharomyces cerevisiae
neopolyoxin C	-	Saccharomyces cerevisiae
nikkomycin Z	-	Candida albicans
nikkomycin Z	specific for Chs1	Saccharomyces cerevisiae
O-methyl pisiferic acid	a diterpene compound isolated from leaves of Chamaecyparis pisifera, inhibition of chitin synthase 1	Candida albicans
O-methyl pisiferic acid	a diterpene compound isolated from leaves of Chamaecyparis pisifera, specifically inhibits Chs2p in a mixed competitive manner versus UDP-N-acetyl-beta-D-glucosamine	Saccharomyces cerevisiae
Polyoxin D	-	Candida albicans
Polyoxin D	-	Saccharomyces cerevisiae

Organism

Organism	UniProt	Comment	Textmining
Candida albicans	P23316	-	-
Saccharomyces cerevisiae	-	strains ECY38-38A and YPH499	-
Saccharomyces cerevisiae	A6ZKY2	chitin synthase 2; strains ECY38-38A and YPH499	-

Source Tissue

Source Tissue	Comment	Organism	Textmining

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
UDP-N-acetyl-D-glucosamine + [4-N-acetyl-beta-D-glucosaminyl-(1-)]n	-	Saccharomyces cerevisiae	UDP + [4-N-acetyl-beta-D-glucosaminyl-(1-)]n+1	-	?
UDP-N-acetyl-D-glucosamine + [4-N-acetyl-beta-D-glucosaminyl-(1-)]n	-	Candida albicans	UDP + [4-N-acetyl-beta-D-glucosaminyl-(1-)]n+1	-	?

Synonyms

Synonyms	Comment	Organism
chitin synthase 1	-	Candida albicans
chitin synthase 2	-	Saccharomyces cerevisiae
CHS1p	-	Saccharomyces cerevisiae
CHS1p	-	Candida albicans
CHS2p	-	Saccharomyces cerevisiae
Chs3p	-	Saccharomyces cerevisiae

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	assay at	Saccharomyces cerevisiae
30	-	assay at	Candida albicans

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	8	assay at	Saccharomyces cerevisiae
7.5	8	assay at	Candida albicans

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.005	-	O-methyl pisiferic acid	versus UDP-N-acetyl-beta-D-glucosamine	Saccharomyces cerevisiae

IC50 Value

IC50 Value	IC50 Value Maximum	Comment	Organism	Inhibitor	Structure
0.0011	-	inhibition of Chs1p	Saccharomyces cerevisiae	neopolyoxin C
0.0058	-	inhibition of Chs2p	Saccharomyces cerevisiae	O-methyl pisiferic acid
0.0059	-	inhibition of Chs3p	Saccharomyces cerevisiae	Polyoxin D
0.0071	-	inhibition of Chs1p	Saccharomyces cerevisiae	Polyoxin D
0.015	-	inhibition of Chs1p	Candida albicans	neopolyoxin C
0.0427	-	inhibition of Chs1p	Candida albicans	Polyoxin D
0.076	-	inhibition of Chs1p	Candida albicans	O-methyl pisiferic acid
0.0886	-	inhibition of Chs2p	Saccharomyces cerevisiae	Polyoxin D
0.171	-	inhibition of Chs1p	Candida albicans	8,20-dihydroxy-9(11),13-abietadien-12-one
0.2264	-	inhibition of Chs2p	Saccharomyces cerevisiae	8,20-dihydroxy-9(11),13-abietadien-12-one
0.355	-	inhibition of Chs2p	Saccharomyces cerevisiae	neopolyoxin C
0.424	-	inhibition of Chs1p	Saccharomyces cerevisiae	O-methyl pisiferic acid
0.44	-	inhibition of Chs1p	Saccharomyces cerevisiae	8,20-dihydroxy-9(11),13-abietadien-12-one

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Release 2023.1 (January 2023)