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Literature summary for 2.4.1.16 extracted from
- Properties of chitin synthetase in isolated chitosomes from yeast cells of Mucor rouxii (1977), J. Biol. Chem., 252, 3338-3343.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| GlcNAc | stimulates | Amylomyces rouxii |  |
General Stability
| General Stability | Organism |
|---|---|
| the zymogen is highly stable | Amylomyces rouxii |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ADP | 6.25 mM | Amylomyces rouxii | |
| AMP | 6.25 mM | Amylomyces rouxii | |
| ATP | 6.25 mM | Amylomyces rouxii | |
| Ca2+ | 5-10 mM | Amylomyces rouxii | |
| Co2+ | 5-10 mM | Amylomyces rouxii | |
| Polyoxin D | - |
Amylomyces rouxii | |
| UDP | - |
Amylomyces rouxii | |
| UMP | 6.25 mM | Amylomyces rouxii | |
| UTP | 6.25 mM | Amylomyces rouxii | |
| Zn2+ | 5-10 mM | Amylomyces rouxii | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| chitosome | - |
Amylomyces rouxii | 45009 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | optimal concentration is 5-20 mM | Amylomyces rouxii | |
| Mg2+ | Mg2+ or Mn2+ required | Amylomyces rouxii | |
| Mn2+ | Mn2+ or Mg2+ required | Amylomyces rouxii | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 700000 | - |
gel filtration | Amylomyces rouxii |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Amylomyces rouxii | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| proteolytic modification | enzyme exists in the zymogenic form and requires proteolytic activation | Amylomyces rouxii |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Amylomyces rouxii |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.01639 | - |
- |
Amylomyces rouxii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-N-acetyl-D-glucosamine + [1,4-(N-acetyl-beta-D-glucosaminyl)]n | - |
Amylomyces rouxii | UDP + [1,4-(N-acetyl-beta-D-glucosaminyl)]n+1 | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | 50 | 5 min, 20-25% loss of activity | Amylomyces rouxii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
- |
Amylomyces rouxii |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00065 | - |
polyoxin | - |
Amylomyces rouxii | |
| 0.4 | - |
UDP | - |
Amylomyces rouxii | |
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Release 2023.1 (January 2023)
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