Activating Compound | Comment | Organism | Structure |
---|---|---|---|
GlcNAc | - |
Saccharomyces cerevisiae |
General Stability | Organism |
---|---|
upon lyophilization, about 70% of the activity is recovered | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.7 | - |
UDP-GlcNAc | - |
Saccharomyces cerevisiae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
570000 | - |
gel filtration, calculation from sedimentation coefficient | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Saccharomyces cerevisiae |
Storage Stability | Organism |
---|---|
-80°C, quite stable for several months | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-N-acetyl-D-glucosamine + [1,4-(N-acetyl-beta-D-glucosaminyl)]n | the enzyme itself is capable both of initiating chitin chains without a primer and of determining their length | Saccharomyces cerevisiae | UDP + [1,4-(N-acetyl-beta-D-glucosaminyl)]n+1 | at low concentrations of UDP-GlcNAc, no insoluble chitin is formed. Instead N-acetylglucosamine is incorporated into water-soluble products | ? |
Subunits | Comment | Organism |
---|---|---|
More | in SDS-PAGE, the purified enzyme shows a major band of 63000 Da and a weaker band at 74000 Da | Saccharomyces cerevisiae |