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Literature summary for 2.4.1.155 extracted from
- High-performance liquid chromatography assays for N-acetylglucosaminyltransferases involved in N- and O-glycan synthesis (1989), Methods Enzymol., 179, 351-397.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.18 | - |
N-acetyl-D-glucosaminyl-1,2-alpha-mannosyl-1,6-beta-mannosyl-O-(CH2)-COOCH3 | - |
Mesocricetus auratus |  |
| 0.25 | - |
N-acetyl-D-glucosamine | - |
Mesocricetus auratus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no requirement for Mn2+ | Gallus gallus | |
| additional information | no requirement for Mn2+ | Mus musculus | |
| additional information | no requirement for Mn2+ | Homo sapiens | |
| additional information | no requirement for Mn2+ | Mesocricetus auratus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-N-acetyl-D-glucosamine + N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3(6)-(N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6(3))-beta-D-mannosyl-1,4-N-acetyl-D-glucosaminyl-R | Gallus gallus | enzyme requires prior action of N-acetylglucosaminyltransferase II which requires prior action of N-acetylglucosaminyltransferase I | UDP + N-acetyl-D-glucosaminyl-1,2-(N-acetyl-D-glucosaminyl-1,6)-1,2-alpha-D-mannosyl-1,3(6)-(N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6(3))-beta-D-mannosyl-1,4-N-acetyl-D-glucosaminyl-R | - |
? | |
| UDP-N-acetyl-D-glucosamine + N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3(6)-(N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6(3))-beta-D-mannosyl-1,4-N-acetyl-D-glucosaminyl-R | Mus musculus | enzyme requires prior action of N-acetylglucosaminyltransferase II which requires prior action of N-acetylglucosaminyltransferase I | UDP + N-acetyl-D-glucosaminyl-1,2-(N-acetyl-D-glucosaminyl-1,6)-1,2-alpha-D-mannosyl-1,3(6)-(N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6(3))-beta-D-mannosyl-1,4-N-acetyl-D-glucosaminyl-R | - |
? | |
| UDP-N-acetyl-D-glucosamine + N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3(6)-(N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6(3))-beta-D-mannosyl-1,4-N-acetyl-D-glucosaminyl-R | Homo sapiens | enzyme requires prior action of N-acetylglucosaminyltransferase II which requires prior action of N-acetylglucosaminyltransferase I | UDP + N-acetyl-D-glucosaminyl-1,2-(N-acetyl-D-glucosaminyl-1,6)-1,2-alpha-D-mannosyl-1,3(6)-(N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6(3))-beta-D-mannosyl-1,4-N-acetyl-D-glucosaminyl-R | - |
? | |
| UDP-N-acetyl-D-glucosamine + N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3(6)-(N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6(3))-beta-D-mannosyl-1,4-N-acetyl-D-glucosaminyl-R | Mesocricetus auratus | enzyme requires prior action of N-acetylglucosaminyltransferase II which requires prior action of N-acetylglucosaminyltransferase I | UDP + N-acetyl-D-glucosaminyl-1,2-(N-acetyl-D-glucosaminyl-1,6)-1,2-alpha-D-mannosyl-1,3(6)-(N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6(3))-beta-D-mannosyl-1,4-N-acetyl-D-glucosaminyl-R | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Gallus gallus | - |
- |
- |
| Homo sapiens | - |
- |
- |
| Mesocricetus auratus | - |
- |
- |
| Mus musculus | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| BW-5147 cell | - |
Mus musculus | - |
| cell culture | - |
Mus musculus | - |
| kidney | - |
Mus musculus | - |
| kidney | - |
Mesocricetus auratus | - |
| myeloid cell line | - |
Homo sapiens | - |
| oviduct | - |
Gallus gallus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-N-acetyl-D-glucosamine + N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3(6)-(N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6(3))-beta-D-mannosyl-1,4-N-acetyl-D-glucosaminyl-R | no substrates are bisected N-glycans | Gallus gallus | UDP + N-acetyl-D-glucosaminyl-1,2-(N-acetyl-D-glucosaminyl-1,6)-1,2-alpha-D-mannosyl-1,3(6)-(N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6(3))-beta-D-mannosyl-1,4-N-acetyl-D-glucosaminyl-R | - |
? | |
| UDP-N-acetyl-D-glucosamine + N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3(6)-(N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6(3))-beta-D-mannosyl-1,4-N-acetyl-D-glucosaminyl-R | no substrates are bisected N-glycans | Mus musculus | UDP + N-acetyl-D-glucosaminyl-1,2-(N-acetyl-D-glucosaminyl-1,6)-1,2-alpha-D-mannosyl-1,3(6)-(N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6(3))-beta-D-mannosyl-1,4-N-acetyl-D-glucosaminyl-R | - |
? | |
| UDP-N-acetyl-D-glucosamine + N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3(6)-(N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6(3))-beta-D-mannosyl-1,4-N-acetyl-D-glucosaminyl-R | no substrates are bisected N-glycans | Homo sapiens | UDP + N-acetyl-D-glucosaminyl-1,2-(N-acetyl-D-glucosaminyl-1,6)-1,2-alpha-D-mannosyl-1,3(6)-(N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6(3))-beta-D-mannosyl-1,4-N-acetyl-D-glucosaminyl-R | - |
? | |
| UDP-N-acetyl-D-glucosamine + N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3(6)-(N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6(3))-beta-D-mannosyl-1,4-N-acetyl-D-glucosaminyl-R | no substrates are bisected N-glycans | Mesocricetus auratus | UDP + N-acetyl-D-glucosaminyl-1,2-(N-acetyl-D-glucosaminyl-1,6)-1,2-alpha-D-mannosyl-1,3(6)-(N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6(3))-beta-D-mannosyl-1,4-N-acetyl-D-glucosaminyl-R | - |
? | |
| UDP-N-acetyl-D-glucosamine + N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3(6)-(N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6(3))-beta-D-mannosyl-1,4-N-acetyl-D-glucosaminyl-R | enzyme requires prior action of N-acetylglucosaminyltransferase II which requires prior action of N-acetylglucosaminyltransferase I | Gallus gallus | UDP + N-acetyl-D-glucosaminyl-1,2-(N-acetyl-D-glucosaminyl-1,6)-1,2-alpha-D-mannosyl-1,3(6)-(N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6(3))-beta-D-mannosyl-1,4-N-acetyl-D-glucosaminyl-R | - |
? | |
| UDP-N-acetyl-D-glucosamine + N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3(6)-(N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6(3))-beta-D-mannosyl-1,4-N-acetyl-D-glucosaminyl-R | enzyme requires prior action of N-acetylglucosaminyltransferase II which requires prior action of N-acetylglucosaminyltransferase I | Mus musculus | UDP + N-acetyl-D-glucosaminyl-1,2-(N-acetyl-D-glucosaminyl-1,6)-1,2-alpha-D-mannosyl-1,3(6)-(N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6(3))-beta-D-mannosyl-1,4-N-acetyl-D-glucosaminyl-R | - |
? | |
| UDP-N-acetyl-D-glucosamine + N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3(6)-(N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6(3))-beta-D-mannosyl-1,4-N-acetyl-D-glucosaminyl-R | enzyme requires prior action of N-acetylglucosaminyltransferase II which requires prior action of N-acetylglucosaminyltransferase I | Homo sapiens | UDP + N-acetyl-D-glucosaminyl-1,2-(N-acetyl-D-glucosaminyl-1,6)-1,2-alpha-D-mannosyl-1,3(6)-(N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6(3))-beta-D-mannosyl-1,4-N-acetyl-D-glucosaminyl-R | - |
? | |
| UDP-N-acetyl-D-glucosamine + N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3(6)-(N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6(3))-beta-D-mannosyl-1,4-N-acetyl-D-glucosaminyl-R | enzyme requires prior action of N-acetylglucosaminyltransferase II which requires prior action of N-acetylglucosaminyltransferase I | Mesocricetus auratus | UDP + N-acetyl-D-glucosaminyl-1,2-(N-acetyl-D-glucosaminyl-1,6)-1,2-alpha-D-mannosyl-1,3(6)-(N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6(3))-beta-D-mannosyl-1,4-N-acetyl-D-glucosaminyl-R | - |
? | |
| UDP-N-acetyl-D-glucosamine + N-acetyl-D-glucosaminyl-1,2-alpha-mannosyl-1,6-beta-glucosyl-O-R | R: hydrophobic group, e.g. (CH2)7-CH3 or (CH2)8-COOCH3 | Mus musculus | UDP + N-acetyl-D-glucosaminyl-1,6-(N-acetyl-D-glucosaminyl-1,2)-alpha-mannosyl-1,6-beta-D-glucosyl-O-R | - |
? | |
| UDP-N-acetyl-D-glucosamine + N-acetyl-D-glucosaminyl-1,2-alpha-mannosyl-1,6-beta-glucosyl-O-R | R: hydrophobic group, e.g. (CH2)7-CH3 or (CH2)8-COOCH3 | Mesocricetus auratus | UDP + N-acetyl-D-glucosaminyl-1,6-(N-acetyl-D-glucosaminyl-1,2)-alpha-mannosyl-1,6-beta-D-glucosyl-O-R | - |
? | |
| UDP-N-acetyl-D-glucosamine + N-acetyl-D-glucosaminyl-1,2-alpha-mannosyl-1,6-beta-mannosyl-O-R | R: hydrophobic group, e.g. (CH2)8-COOCH3 | Mus musculus | UDP + N-acetyl-D-glucosaminyl-1,6-(N-acetyl-D-glucosaminyl-1,2)-alpha-mannosyl-1,6-beta-D-mannosyl-O-R | - |
? | |
| UDP-N-acetyl-D-glucosamine + N-acetyl-D-glucosaminyl-1,2-alpha-mannosyl-1,6-beta-mannosyl-O-R | R: hydrophobic group, e.g. (CH2)8-COOCH3 | Mesocricetus auratus | UDP + N-acetyl-D-glucosaminyl-1,6-(N-acetyl-D-glucosaminyl-1,2)-alpha-mannosyl-1,6-beta-D-mannosyl-O-R | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Gallus gallus |
| 37 | - |
assay at | Mus musculus |
| 37 | - |
assay at | Homo sapiens |
| 37 | - |
assay at | Mesocricetus auratus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
around | Mesocricetus auratus |
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