Cloned (Comment) | Organism |
---|---|
full-length form of human FUT9 and a truncated form containing sFUT9 the catalytic domain of the enzyme are overexpressed in Spodoptera frugiperda (Sf9) insect cells using the signal sequence of human interleukin 2 for efficient secretion, high activity of the trunctated, soluble form sFUT9 in the supernatant of Sf9 cells, sFUT9 fucosylates sialylated and non-sialylated glycoproteins | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37800 | - |
after PNGaseF treatment | Homo sapiens |
41300 | - |
SDS-PAGE, sFUT9 | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GDP-fucose + asialo human erythropoietin | - |
Homo sapiens | GDP + fucosylated asialo human erythropoietin | - |
? | |
GDP-fucose + bovine asialofetuin | - |
Homo sapiens | GDP + fucosylated bovine asialofetuin | - |
? | |
GDP-fucose + Fucalpha1-2Galbeta1-4GlcNAc-O-(CH2)3NHCO(CH2)5-NH-biotin | - |
Homo sapiens | GDP + Fucalpha1-2Galbeta1-4(Fucalpha1-3)GlcNAc-O-(CH2)3NHCO(CH2)5-biotin | - |
? | |
GDP-fucose + Galbeta1-4Glc-NAc-O-(CH2)3NHCO(CH2)5-NH-biotin | - |
Homo sapiens | GDP + Galbeta1-4(Fucalpha1-3)GlcNAc-O-(CH2)3NHCO(CH2)5-NH-biotin | - |
? | |
GDP-fucose + Galbeta1-4GlcNAc-O-(CH2)3NHCO(CH2)5-NH-biotin | sFUT9 efficiently fucosylates type II acceptors but not the corresponding sialylated acceptors, and only very poorly the type I (Galbeta3GlcNAc-R) related acceptors | Homo sapiens | GDP + Galbeta1-4(Fucalpha1-3)GlcNAc-O-(CH2)3NHCO(CH2)5-NH-biotin | - |
? | |
GDP-fucose + human erythropoietin | - |
Homo sapiens | GDP + fucosylated human erythropoietin | - |
? |
Synonyms | Comment | Organism |
---|---|---|
alpha 3-fucosyltransferase IX | - |
Homo sapiens |
Fut9 | - |
Homo sapiens |
sFUT9 | truncated form | Homo sapiens |