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Literature summary for 2.4.1.150 extracted from

  • Sakamoto, Y.; Taguchi, T.; Tano, Y.; Ogawa, T.; Leppänen, A.; Kinnunen, M.; Aitio, O.; Parmanne, P.; Renkonen, O.; Taniguchi, N.
    Purification and characterization of UDP-GlcNAc:Galb1-4-GlcNAcb1-3*Galb1-4Glc(NAc)-R(GlcNAc to *Gal) beta1,6N-acetylglucosaminyltransferase from hog small intestine (1998), J. Biol. Chem., 273, 27625-27632.
    View publication on PubMed

General Stability

General Stability Organism
bovine serum albumin, 2 mg/ml, preserves enzyme activity at 37°C, especially the highly purified enzyme fraction Sus scrofa

Inhibitors

Inhibitors Comment Organism Structure
AMP 10% inhibition Sus scrofa
CuCl2
-
Sus scrofa
GDP 19% inhibition Sus scrofa
NiCl2
-
Sus scrofa
UDP most potent inhibitor of donor substrate analogues, complete inhibition Sus scrofa
UDP-galactose 42% inhibition Sus scrofa
UDP-glucose 51% inhibition Sus scrofa
UDP-glucuronic acid 36% inhibition Sus scrofa
UDP-hexanolamine 66% inhibition Sus scrofa
UMP 41% inhibition Sus scrofa
UTP most potent inhibitor of donor substrate analogues, complete inhibition Sus scrofa
ZnCl2
-
Sus scrofa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.96
-
pyridylaminated lacto-N-neotetraose
-
Sus scrofa
2.59
-
UDP-N-acetyl-D-glucosamine
-
Sus scrofa

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome enzyme is concentrated in the microsome fraction Sus scrofa
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
additional information no requirement for any divalent metal ions Sus scrofa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
76000
-
x * 76000, SDS-PAGE under nonreducing conditions Sus scrofa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-N-acetyl-D-glucosamine + poly-N-acetyllactosamine Sus scrofa responsible for the formation of the beta1-6-branched poly-N-acetyllactosamine structure, involved in generating branches to central positions of preformed as well as growing polylactosamine chains but not in synthesizing the distal branches to growing chains ?
-
?

Organism

Organism UniProt Comment Textmining
Sus scrofa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
210000fold, cIGnT6 Sus scrofa

Source Tissue

Source Tissue Comment Organism Textmining
small intestine highest enzyme activity per tissue protein Sus scrofa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.058
-
-
Sus scrofa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information 2 types of branching enzyme activities: cIGnT6 generates beta-1,6-N-acetylglucosaminyl branches at the central galactose residue, and dIGnT6 acts on peridistal galactose residues Sus scrofa ?
-
?
additional information absolute requirement of at least a complete Galbeta(1-4)GlcNAc residue bound to position 3 of the acceptor Gal residues, i.e. it is capable of acting only on the Gal residues of internal Galbeta(1-4)GlcNAc units, no substrates: pyridylaminated GlcNAcbeta(1-3)Galbeta(1-4)Glc and pyridylaminated Galbeta(1-3)GlcNAcbeta(1-3)Galbeta(1-4)Glc, 4 major groups according to their acceptor specificities: blood group I structure: IGnT6, core 2 in O-glycans: C2GnT6, core 4 in O-glycans: C4GnT6, 2,6-branched N-linked core: GnT V Sus scrofa ?
-
?
UDP-N-acetyl-D-glucosamine + Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)GlcNAc cIGnT6 Sus scrofa UDP + Galbeta(1-4)GlcNAcbeta(1-3)(GlcNAcbeta(1-6))Galbeta(1-4)GlcNAc
-
?
UDP-N-acetyl-D-glucosamine + Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)GlcNAc cIGnT6 Sus scrofa UDP + Galbeta(1-4)GlcNAcbeta(1-3)(GlcNAcbeta(1-6))Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)GlcNAc cIGnT6, 60% of the heptasaccharide product, 40% of the heptasaccharide product is Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)GlcNAcbeta(1-3)(GlcNAcbeta(1-6))Galbeta(1-4)GlcNAc, 95% heptasaccharide and 5% di-branched octasaccharide product ?
UDP-N-acetyl-D-glucosamine + GlcNAcbeta(1-3)Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)GlcNAc cIGnT6 Sus scrofa UDP + GlcNAcbeta(1-3)Galbeta(1-4)GlcNAcbeta(1-3)(GlcNAcbeta(1-6))Galbeta(1-4)GlcNAc
-
?
UDP-N-acetyl-D-glucosamine + poly-N-acetyllactosamine cIGnT6 Sus scrofa ?
-
?
UDP-N-acetyl-D-glucosamine + poly-N-acetyllactosamine forms branches in poly-N-acetyllactosamines Sus scrofa ?
-
?
UDP-N-acetyl-D-glucosamine + poly-N-acetyllactosamine responsible for the formation of the beta1-6-branched poly-N-acetyllactosamine structure, involved in generating branches to central positions of preformed as well as growing polylactosamine chains but not in synthesizing the distal branches to growing chains Sus scrofa ?
-
?
UDP-N-acetyl-D-glucosamine + pyridylaminated Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)Glc i.e. pyridylaminated lacto-N-neotetraose, cIGnT6 Sus scrofa UDP + pyridylaminated Galbeta(1-4)GlcNAcbeta(1-3)(GlcNAcbeta(1-6))Galbeta(1-4)Glc
-
?

Subunits

Subunits Comment Organism
? x * 76000, SDS-PAGE under nonreducing conditions Sus scrofa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Sus scrofa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Sus scrofa