General Stability | Organism |
---|---|
bovine serum albumin, 2 mg/ml, preserves enzyme activity at 37°C, especially the highly purified enzyme fraction | Sus scrofa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
AMP | 10% inhibition | Sus scrofa | |
CuCl2 | - |
Sus scrofa | |
GDP | 19% inhibition | Sus scrofa | |
NiCl2 | - |
Sus scrofa | |
UDP | most potent inhibitor of donor substrate analogues, complete inhibition | Sus scrofa | |
UDP-galactose | 42% inhibition | Sus scrofa | |
UDP-glucose | 51% inhibition | Sus scrofa | |
UDP-glucuronic acid | 36% inhibition | Sus scrofa | |
UDP-hexanolamine | 66% inhibition | Sus scrofa | |
UMP | 41% inhibition | Sus scrofa | |
UTP | most potent inhibitor of donor substrate analogues, complete inhibition | Sus scrofa | |
ZnCl2 | - |
Sus scrofa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.96 | - |
pyridylaminated lacto-N-neotetraose | - |
Sus scrofa | |
2.59 | - |
UDP-N-acetyl-D-glucosamine | - |
Sus scrofa |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microsome | enzyme is concentrated in the microsome fraction | Sus scrofa | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | no requirement for any divalent metal ions | Sus scrofa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
76000 | - |
x * 76000, SDS-PAGE under nonreducing conditions | Sus scrofa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-N-acetyl-D-glucosamine + poly-N-acetyllactosamine | Sus scrofa | responsible for the formation of the beta1-6-branched poly-N-acetyllactosamine structure, involved in generating branches to central positions of preformed as well as growing polylactosamine chains but not in synthesizing the distal branches to growing chains | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sus scrofa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
210000fold, cIGnT6 | Sus scrofa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
small intestine | highest enzyme activity per tissue protein | Sus scrofa | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.058 | - |
- |
Sus scrofa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | 2 types of branching enzyme activities: cIGnT6 generates beta-1,6-N-acetylglucosaminyl branches at the central galactose residue, and dIGnT6 acts on peridistal galactose residues | Sus scrofa | ? | - |
? | |
additional information | absolute requirement of at least a complete Galbeta(1-4)GlcNAc residue bound to position 3 of the acceptor Gal residues, i.e. it is capable of acting only on the Gal residues of internal Galbeta(1-4)GlcNAc units, no substrates: pyridylaminated GlcNAcbeta(1-3)Galbeta(1-4)Glc and pyridylaminated Galbeta(1-3)GlcNAcbeta(1-3)Galbeta(1-4)Glc, 4 major groups according to their acceptor specificities: blood group I structure: IGnT6, core 2 in O-glycans: C2GnT6, core 4 in O-glycans: C4GnT6, 2,6-branched N-linked core: GnT V | Sus scrofa | ? | - |
? | |
UDP-N-acetyl-D-glucosamine + Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)GlcNAc | cIGnT6 | Sus scrofa | UDP + Galbeta(1-4)GlcNAcbeta(1-3)(GlcNAcbeta(1-6))Galbeta(1-4)GlcNAc | - |
? | |
UDP-N-acetyl-D-glucosamine + Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)GlcNAc | cIGnT6 | Sus scrofa | UDP + Galbeta(1-4)GlcNAcbeta(1-3)(GlcNAcbeta(1-6))Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)GlcNAc | cIGnT6, 60% of the heptasaccharide product, 40% of the heptasaccharide product is Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)GlcNAcbeta(1-3)(GlcNAcbeta(1-6))Galbeta(1-4)GlcNAc, 95% heptasaccharide and 5% di-branched octasaccharide product | ? | |
UDP-N-acetyl-D-glucosamine + GlcNAcbeta(1-3)Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)GlcNAc | cIGnT6 | Sus scrofa | UDP + GlcNAcbeta(1-3)Galbeta(1-4)GlcNAcbeta(1-3)(GlcNAcbeta(1-6))Galbeta(1-4)GlcNAc | - |
? | |
UDP-N-acetyl-D-glucosamine + poly-N-acetyllactosamine | cIGnT6 | Sus scrofa | ? | - |
? | |
UDP-N-acetyl-D-glucosamine + poly-N-acetyllactosamine | forms branches in poly-N-acetyllactosamines | Sus scrofa | ? | - |
? | |
UDP-N-acetyl-D-glucosamine + poly-N-acetyllactosamine | responsible for the formation of the beta1-6-branched poly-N-acetyllactosamine structure, involved in generating branches to central positions of preformed as well as growing polylactosamine chains but not in synthesizing the distal branches to growing chains | Sus scrofa | ? | - |
? | |
UDP-N-acetyl-D-glucosamine + pyridylaminated Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)Glc | i.e. pyridylaminated lacto-N-neotetraose, cIGnT6 | Sus scrofa | UDP + pyridylaminated Galbeta(1-4)GlcNAcbeta(1-3)(GlcNAcbeta(1-6))Galbeta(1-4)Glc | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 76000, SDS-PAGE under nonreducing conditions | Sus scrofa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Sus scrofa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Sus scrofa |