Application | Comment | Organism |
---|---|---|
synthesis | cIGnT6 transfers multiple GlcNAc branches to long linear polylactosamines, a prerequisite for improving enzyme-assisted in vitro synthesis of a type of multivalent sialyl Lewis x glycans that are high affinity inhibitors of lymphocyte L-selectin, enzyme allows general polylactosamine synthesis | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
IGnT6 is cloned, sequenced and cDNA encoding cIGnT6 in PA-1 cells is expressed in SF9 insect cells as protein fused to glutathione S-transferase | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-N-acetyl-D-glucosamine + poly-N-acetyllactosamine | Homo sapiens | responsible for the conversion of linear to branched polylactosamines, cIGnT6 actions at central rather than peridistal galactose residues of linear polylactosamines in the biosynthesis of blood group I antigens | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | recombinant enzyme, expressed in SF9 insect cells as fusion protein with glutathione S-transferase has 5 potential N-glycosylation sites | Homo sapiens |
Purification (Comment) | Organism |
---|---|
purification of recombinant enzyme, expressed in SF9 insect cells as protein fused to glutathione S-transferase | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
PA-1 cell | cIGnT6, embryonal carcinoma cell line | Homo sapiens | - |
Storage Stability | Organism |
---|---|
-20°C, recombinant enzyme, a few days, stable | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | 2 types of branching enzyme activities: cIGnT6 generates beta-1,6-N-acetylglucosaminyl branches at the central galactose residue, and dIGnT6 acts on peridistal galactose residues | Homo sapiens | ? | - |
? | |
additional information | no substrate of cIGnT6: GlcNAcbeta(1-3)Galbeta(1-4)GlcNAc, Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)(Fucalpha(1-3))GlcNAc, no transfer of GlcNAc to substrates with alpha1-3-fucosyl residues and to midchain galactoses that belongs to Lewis x determinants | Homo sapiens | ? | - |
? | |
UDP-N-acetyl-D-glucosamine + Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)GlcNAc | cIGnT | Homo sapiens | UDP + Galbeta(1-4)GlcNAcbeta(1-3)(GlcNAcbeta(1-6))Galbeta(1-4)GlcNAc | - |
? | |
UDP-N-acetyl-D-glucosamine + Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)GlcNAc | cIGnT | Homo sapiens | UDP + (GlcNAc)1-Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)GlcNAc | also as product: (GlcNAc)2-Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)GlcNAc, cIGnT6 generates in a partial reaction nona- and decasaccharide products, which represent mixtures of isomers carrying one or two GlcNAc-branches on the linear octasaccharide acceptor | ? | |
UDP-N-acetyl-D-glucosamine + poly-N-acetyllactosamine | forms branches in poly-N-acetyllactosamines | Homo sapiens | ? | - |
? | |
UDP-N-acetyl-D-glucosamine + poly-N-acetyllactosamine | cIGnT6 transfers one or multiple GlcNAc branches to midchain galactoses of long linear polylactosamines | Homo sapiens | ? | - |
? | |
UDP-N-acetyl-D-glucosamine + poly-N-acetyllactosamine | responsible for the conversion of linear to branched polylactosamines, cIGnT6 actions at central rather than peridistal galactose residues of linear polylactosamines in the biosynthesis of blood group I antigens | Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 67000-74000, glycosylated protein fused to glutathione S-transferase, size heterogenicity is at least partially due to differences in N-glycosylation, SDS-PAGE | Homo sapiens |