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Literature summary for 2.4.1.15 extracted from
- Aggregation dependent enhancement of trehalose-6-phosphate synthase activity in Saccharomyces cerevisiae (2008), Biochim. Biophys. Acta, 1780, 289-297.
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 59000 | - |
estimated by HPGPLC using a Ultropak G2000SW column and by SDS-PAGE | Saccharomyces cerevisiae |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-alpha-D-glucose + D-glucose 6-phosphate | Saccharomyces cerevisiae | - |
UDP + alpha,alpha-trehalose 6-phosphate | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| crude extract is prepared, the enzyme is isolated by ammonium sulfate fractionation and high performance gel permeation liquid chromatography, using a HiLoad 16/60 Superdex 200 and a Ultropak TSK G2000SW column | Saccharomyces cerevisiae |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
the activity of trehalose-6-phosphate synthase is concentration dependent | Saccharomyces cerevisiae |
| 0.01 | - |
preparation of trehalose-6-phosphate synthase from Saccharomyces cerevisiae, preparation step soluble supernatant | Saccharomyces cerevisiae |
| 0.49 | - |
preparation of trehalose-6-phosphate synthase from Saccharomyces cerevisiae, preparation step ammonimum sulfate fractionation | Saccharomyces cerevisiae |
| 0.74 | - |
preparation of trehalose-6-phosphate synthase from Saccharomyces cerevisiae, preparation step HPGPLC on TSK G2000SW | Saccharomyces cerevisiae |
| 1.58 | - |
preparation of trehalose-6-phosphate synthase from Saccharomyces cerevisiae, preparation step HPGPLC on HiLoad 16/60 Superdex 200 | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-alpha-D-glucose + D-glucose 6-phosphate | - |
Saccharomyces cerevisiae | UDP + alpha,alpha-trehalose 6-phosphate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| alpha,alpha-trehalose-phosphate synthase (UDP-forming) | - |
Saccharomyces cerevisiae |
| TPS | - |
Saccharomyces cerevisiae |
| trehalose-6-phosphate synthase | key subunit of the trehalose synthase complex | Saccharomyces cerevisiae |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
activity assay | Saccharomyces cerevisiae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
activity assay | Saccharomyces cerevisiae |
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Release 2023.1 (January 2023)
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