Application | Comment | Organism |
---|---|---|
agriculture | the results demonstrate that engineering trehalose metabolism with a yeast TPS-TPP bifunctional enzyme confers multiple stress protection in plants, comprising a potential tool to improve stress-tolerance in crops | Saccharomyces cerevisiae |
Cloned (Comment) | Organism |
---|---|
a translational trehalose-6-phosphate synthase - trehalose-6-phosphate phosphatase, TPS-TPP, gene-fusion is constructed and subcloned into the pBluescript SK vector, into the yeast expression vector pSal4, and into the pBin19 vector for transformation of Arabidopsis thaliana | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-alpha-D-glucose + D-glucose 6-phosphate | Saccharomyces cerevisiae | - |
UDP + alpha,alpha-trehalose 6-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.011 | - |
TPS specific activity in glucose, yeast mutant strain tps2delta + pSAL4 | Saccharomyces cerevisiae |
0.013 | - |
TPS specific activity in galactose, yeast mutant strain tps1delta_tps2delta + pSAL4::TPS1 | Saccharomyces cerevisiae |
0.02 | - |
TPS specific activity in glucose, yeast mutant strain tps1delta_tps2delta + pSAL4::TPS1 | Saccharomyces cerevisiae |
0.055 | - |
TPS specific activity in galactose, yeast wild type + pSAL4 | Saccharomyces cerevisiae |
0.063 | - |
TPS specific activity in galactose, yeast mutant strain tps2delta + pSAL4::TPS2 | Saccharomyces cerevisiae |
0.067 | - |
TPS specific activity in glucose, yeast mutant strain tps2delta + pSAL4::TPS2 | Saccharomyces cerevisiae |
0.073 | - |
TPS specific activity in glucose, yeast mutant strain tps2delta + pSAL4::TPS1-TPS2 | Saccharomyces cerevisiae |
0.078 | - |
TPS specific activity in galactose, yeast mutant strain tps2delta + pSAL4::TPS1-TPS2 | Saccharomyces cerevisiae |
0.079 | - |
TPS specific activity in glucose, yeast wild type + pSAL4 | Saccharomyces cerevisiae |
0.081 | - |
TPS specific activity in galactose, yeast mutant strain tps1delta + pSAL4::TPS1-TPS2 | Saccharomyces cerevisiae |
0.086 | - |
TPS specific activity in glucose, yeast mutant strain tps1delta + pSAL4::TPS1-TPS2 | Saccharomyces cerevisiae |
0.088 | - |
TPS specific activity in galactose, yeast mutant strain tps1delta_tps2delta + pSAL4::TPS1-TPS2 | Saccharomyces cerevisiae |
0.093 | - |
TPS specific activity in glucose, yeast mutant strain tps1delta_tps2delta + pSAL4::TPS1-TPS2 | Saccharomyces cerevisiae |
0.144 | - |
TPS specific activity in glucose, yeast mutant strain tps1delta + pSAL4::TPS1 | Saccharomyces cerevisiae |
0.163 | - |
TPS specific activity in galactose, yeast mutant strain tps1delta + pSAL4::TPS1 | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-alpha-D-glucose + D-glucose 6-phosphate | - |
Saccharomyces cerevisiae | UDP + alpha,alpha-trehalose 6-phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
TPS1 | - |
Saccharomyces cerevisiae |
trehalose-6-phosphate synthase | - |
Saccharomyces cerevisiae |