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Literature summary for 2.4.1.15 extracted from

  • Gibson, R.P.; Tarling, C.A.; Roberts, S.; Withers, S.G.; Davies, G.J.
    The donor subsite of trehalose-6-phosphate synthase: binary complexes with UDP-glucose and UDP-2-deoxy-2-fluoro-glucose at 2 A resolution (2004), J. Biol. Chem., 279, 1950-1955.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
purified recombinant enzyme complexed with either substrate UDP-glucose or the inactive substrate analogue UDP-2-deoxy-2-fluoroglucose, hanging drop vapour diffusion method, 100 nl droplets of 10 mg/ml protein in 20 mM Tris-HCl, pH 8.0, and 200 mM NaCl, with either 25 mM UDP-glucose and 50 mM 1-deoxy-glucose 6-phosphate or 25 mM UDP-2-deoxy-2-fluoroglucose and 20 mM glucose 6-phosphate, precipitant solution: 30% w/v PEG 4000, 200 mM ammonium acetate, 100 mM Tris-HCl, pH 8.0, 36 h, 25% ethylene glycol as cryoprotectant, X-ray diffraction structure determination and analysis at 2.0 A resolution Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-alpha-D-glucose + D-glucose 6-phosphate Escherichia coli step in trehalose biosynthesis UDP + alpha,alpha-trehalose 6-phosphate
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?

Organism

Organism UniProt Comment Textmining
Escherichia coli
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-
-

Reaction

Reaction Comment Organism Reaction ID
UDP-alpha-D-glucose + D-glucose 6-phosphate = UDP + alpha,alpha-trehalose 6-phosphate reaction mechanism Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information donor subsite interactions, enzyme-substrate binding conformation, the N-terminal loop, residues 9-22, is responsible for the more relaxed coformation of the binary enzyme-UDP-sugar complex, overview Escherichia coli ?
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?
UDP-alpha-D-glucose + D-glucose 6-phosphate step in trehalose biosynthesis Escherichia coli UDP + alpha,alpha-trehalose 6-phosphate
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?
UDP-alpha-D-glucose + D-glucose 6-phosphate with retention of the anomeric configuration of the UDP-sugar donor, reaction stereochemistry Escherichia coli UDP + alpha,alpha-trehalose 6-phosphate
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?

Subunits

Subunits Comment Organism
More two domain enzyme Escherichia coli

Synonyms

Synonyms Comment Organism
OtsA
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Escherichia coli
trehalose-6-phosphate synthase
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Escherichia coli