Activating Compound | Comment | Organism | Structure |
---|---|---|---|
D-fructose | - |
Selaginella lepidophylla | |
D-fructose 6-phosphate | - |
Selaginella lepidophylla | |
D-glucose | - |
Selaginella lepidophylla | |
additional information | no effect by sucrose and trehalose up tp 0.4 M | Selaginella lepidophylla |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | no effect by sucrose and trehalose up to 0.4 M, no substrate and product inhibition | Selaginella lepidophylla | |
proline | - |
Selaginella lepidophylla |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | activates at physiological concentrations | Selaginella lepidophylla | |
K+ | activates maximal at 50 mM by 176%, 160% at 400 mM | Selaginella lepidophylla | |
Mg2+ | activates at low concentration up to 5 mM | Selaginella lepidophylla | |
additional information | no effect by Na+ | Selaginella lepidophylla |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
3 aggregation forms of 230, 440, and 660 kDa contain polypeptides of 50, 67, and 115 kDa polypeptides, only the 440 kDa form is catalytically active, native PAGE | Selaginella lepidophylla |
115000 | - |
4 * 115000, SDS-PAGE | Selaginella lepidophylla |
440000 | - |
native PAGE | Selaginella lepidophylla |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-alpha-D-glucose + D-glucose 6-phosphate | Selaginella lepidophylla | step in trehalose biosynthesis | UDP + alpha,alpha-trehalose 6-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Selaginella lepidophylla | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
no glycoprotein | - |
Selaginella lepidophylla |
Purification (Comment) | Organism |
---|---|
native enzyme 50fold by ammonium sulfate fractionation, gel filtration, and nickel-resin affinity chromatography | Selaginella lepidophylla |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
plant | - |
Selaginella lepidophylla | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
7.05 | - |
purified enzyme | Selaginella lepidophylla |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | substrate specificity, no activity with trehalose, maltose, or fructose as acceptor substrates | Selaginella lepidophylla | ? | - |
? | |
UDP-alpha-D-glucose + D-glucose 6-phosphate | step in trehalose biosynthesis | Selaginella lepidophylla | UDP + alpha,alpha-trehalose 6-phosphate | - |
? | |
UDP-alpha-D-glucose + D-glucose 6-phosphate | the enzyme is highly specific for UDP-glucose and D-glucose 6-phosphate | Selaginella lepidophylla | UDP + alpha,alpha-trehalose 6-phosphate | - |
? | |
UDP-glucose + D-fructose 6-phosphate | 16% of the activity with D-glucose 6-phosphate | Selaginella lepidophylla | UDP + ? | - |
? | |
UDP-glucose + D-galactose | 17% of the activity with D-glucose 6-phosphate | Selaginella lepidophylla | UDP + ? | - |
? | |
UDP-glucose + D-glucose | 28% of the activity with D-glucose 6-phosphate | Selaginella lepidophylla | UDP + ? | - |
? | |
UDP-glucose + lactose | 10% of the activity with D-glucose 6-phosphate | Selaginella lepidophylla | UDP + ? | - |
? | |
UDP-glucose + sucrose | 19% of the activity with D-glucose 6-phosphate | Selaginella lepidophylla | UDP + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 115000, SDS-PAGE | Selaginella lepidophylla |
Synonyms | Comment | Organism |
---|---|---|
TPS | - |
Selaginella lepidophylla |
trehalose 6-phosphate synthase | - |
Selaginella lepidophylla |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
assay at | Selaginella lepidophylla |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Selaginella lepidophylla |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 10 | stable | Selaginella lepidophylla |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Selaginella lepidophylla | 115 kDa enzyme polypeptide, isoelectric focusing | - |
4.55 |
Selaginella lepidophylla | 67 kDa enzyme polypeptide, isoelectric focusing | - |
4.69 |
Selaginella lepidophylla | 50 kDa enzyme polypeptide, isoelectric focusing | - |
4.83 |