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Literature summary for 2.4.1.15 extracted from

  • Valenzuela-Soto, E.M.; Marquez-Escalante, J.A.; Iturriaga, G.; Figueroa-Soto, C.G.
    Trehalose 6-phosphate synthase from Selaginella lepidophylla: purification and properties (2004), Biochem. Biophys. Res. Commun., 313, 314-319.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
D-fructose
-
Selaginella lepidophylla
D-fructose 6-phosphate
-
Selaginella lepidophylla
D-glucose
-
Selaginella lepidophylla
additional information no effect by sucrose and trehalose up tp 0.4 M Selaginella lepidophylla

Inhibitors

Inhibitors Comment Organism Structure
additional information no effect by sucrose and trehalose up to 0.4 M, no substrate and product inhibition Selaginella lepidophylla
proline
-
Selaginella lepidophylla

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activates at physiological concentrations Selaginella lepidophylla
K+ activates maximal at 50 mM by 176%, 160% at 400 mM Selaginella lepidophylla
Mg2+ activates at low concentration up to 5 mM Selaginella lepidophylla
additional information no effect by Na+ Selaginella lepidophylla

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
3 aggregation forms of 230, 440, and 660 kDa contain polypeptides of 50, 67, and 115 kDa polypeptides, only the 440 kDa form is catalytically active, native PAGE Selaginella lepidophylla
115000
-
4 * 115000, SDS-PAGE Selaginella lepidophylla
440000
-
native PAGE Selaginella lepidophylla

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-alpha-D-glucose + D-glucose 6-phosphate Selaginella lepidophylla step in trehalose biosynthesis UDP + alpha,alpha-trehalose 6-phosphate
-
?

Organism

Organism UniProt Comment Textmining
Selaginella lepidophylla
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
no glycoprotein
-
Selaginella lepidophylla

Purification (Commentary)

Purification (Comment) Organism
native enzyme 50fold by ammonium sulfate fractionation, gel filtration, and nickel-resin affinity chromatography Selaginella lepidophylla

Source Tissue

Source Tissue Comment Organism Textmining
plant
-
Selaginella lepidophylla
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
7.05
-
purified enzyme Selaginella lepidophylla

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information substrate specificity, no activity with trehalose, maltose, or fructose as acceptor substrates Selaginella lepidophylla ?
-
?
UDP-alpha-D-glucose + D-glucose 6-phosphate step in trehalose biosynthesis Selaginella lepidophylla UDP + alpha,alpha-trehalose 6-phosphate
-
?
UDP-alpha-D-glucose + D-glucose 6-phosphate the enzyme is highly specific for UDP-glucose and D-glucose 6-phosphate Selaginella lepidophylla UDP + alpha,alpha-trehalose 6-phosphate
-
?
UDP-glucose + D-fructose 6-phosphate 16% of the activity with D-glucose 6-phosphate Selaginella lepidophylla UDP + ?
-
?
UDP-glucose + D-galactose 17% of the activity with D-glucose 6-phosphate Selaginella lepidophylla UDP + ?
-
?
UDP-glucose + D-glucose 28% of the activity with D-glucose 6-phosphate Selaginella lepidophylla UDP + ?
-
?
UDP-glucose + lactose 10% of the activity with D-glucose 6-phosphate Selaginella lepidophylla UDP + ?
-
?
UDP-glucose + sucrose 19% of the activity with D-glucose 6-phosphate Selaginella lepidophylla UDP + ?
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 115000, SDS-PAGE Selaginella lepidophylla

Synonyms

Synonyms Comment Organism
TPS
-
Selaginella lepidophylla
trehalose 6-phosphate synthase
-
Selaginella lepidophylla

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
assay at Selaginella lepidophylla

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Selaginella lepidophylla

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 10 stable Selaginella lepidophylla

pI Value

Organism Comment pI Value Maximum pI Value
Selaginella lepidophylla 115 kDa enzyme polypeptide, isoelectric focusing
-
4.55
Selaginella lepidophylla 67 kDa enzyme polypeptide, isoelectric focusing
-
4.69
Selaginella lepidophylla 50 kDa enzyme polypeptide, isoelectric focusing
-
4.83