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Literature summary for 2.4.1.15 extracted from
- Trehalose 6-phosphate synthase from Dictyostelium discoideum: partial purification and characterization of the enzyme from young sorocarps (1979), Arch. Biochem. Biophys., 196, 121-133.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| chondroitin sulfate | activation | Dictyostelium discoideum |  |
| EDTA | activation | Dictyostelium discoideum | |
| heparin | activation | Dictyostelium discoideum | |
| Tween 80 | slight activation | Dictyostelium discoideum | |
General Stability
| General Stability | Organism |
|---|---|
| stable to at least 4 successive freeze/thaw cycles | Dictyostelium discoideum |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cemusol NPT-12 | weak inhibition | Dictyostelium discoideum | |
| Triton X-100 | weak inhibition | Dictyostelium discoideum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | complex bimodal kinetics | Dictyostelium discoideum |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| K+ | maximal activation at 400 mM KCl | Dictyostelium discoideum | |
| Mg2+ | activation | Dictyostelium discoideum | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Dictyostelium discoideum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Dictyostelium discoideum |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| UDP-alpha-D-glucose + D-glucose 6-phosphate = UDP + alpha,alpha-trehalose 6-phosphate | complex bimodal kinetics | Dictyostelium discoideum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| sporocarp | - |
Dictyostelium discoideum | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 6.179 | - |
- |
Dictyostelium discoideum |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -12°C, stable for at least 11 weeks | Dictyostelium discoideum |
| 4-6°C, 2 d, enzyme retains 75% of its activity after 2 d and 25% of its activity after 3 d | Dictyostelium discoideum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ADP-alpha-D-glucose + D-glucose 6-phosphate | 13.4% of the activity with GDPglucose | Dictyostelium discoideum | ADP + alpha,alpha-trehalose 6-phosphate | - |
? | |
| GDP-glucose + D-glucose 6-phosphate | 18.4% of the activity with UDP-glucose | Dictyostelium discoideum | trehalose 6-phosphate + GDP | - |
? | |
| TDP-glucose + glucose 6-phosphate | 15.9% of the activity with UDPglucose | Dictyostelium discoideum | trehalose 6-phosphate + TDP | - |
? | |
| UDPglucose + glucose 6-phosphate | absolute specificity for glucose 6-phosphate | Dictyostelium discoideum | trehalose 6-phosphate + UDP | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 48 | - |
5 min, 44% of its original activity is recovered | Dictyostelium discoideum |
| 55 | - |
5 min, 5% of its original activity is recovered | Dictyostelium discoideum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
- |
Dictyostelium discoideum |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 8 | pH 5.5: about 75% of maximal activity, pH 8.0: about 70% of maximal activity | Dictyostelium discoideum |
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