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Literature summary for 2.4.1.15 extracted from

  • Bell, W.; Klaassen, P.; Ohnacker, M.; Boller, T.; Herweijer, M.; Schoppink, P.; van der Zee, P.; Wiemken, A.
    Characterization of the 56-kDa subunit of yeast trehalose-6-phosphate synthase and cloning of its gene reveal its identity with the product of CIF1, a regulator of carbon catabolite inactivation (1992), Eur. J. Biochem., 209, 951-959.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
isozyme TPS1 Saccharomyces cerevisiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55000
-
x * 55000, isozyme TPS1, x * 100000, isozyme TPS2, x * 105000, isozyme TPS3, SDS-PAGE Saccharomyces cerevisiae
56200
-
x * 56200, calculation from nucleotide sequence Saccharomyces cerevisiae
100000
-
x * 55000, isozyme TPS1, x * 100000, isozyme TPS2, x * 105000, isozyme TPS3, SDS-PAGE Saccharomyces cerevisiae
105000
-
x * 55000, isozyme TPS1, x * 100000, isozyme TPS2, x * 105000, isozyme TPS3, SDS-PAGE Saccharomyces cerevisiae
630000
-
gel filtration Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDPglucose + glucose 6-phosphate Saccharomyces cerevisiae key enzyme for biosynthesis of trehalose, the major soluble carbohydrate in resting cells trehalose 6-phosphate + UDP
-
?
UDPglucose + glucose 6-phosphate Saccharomyces cerevisiae C13-ABYS86 key enzyme for biosynthesis of trehalose, the major soluble carbohydrate in resting cells trehalose 6-phosphate + UDP
-
?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
lacking vacuolar proteases
-
Saccharomyces cerevisiae C13-ABYS86
-
lacking vacuolar proteases
-

Purification (Commentary)

Purification (Comment) Organism
-
Saccharomyces cerevisiae

Source Tissue

Source Tissue Comment Organism Textmining

Storage Stability

Storage Stability Organism
-20°C, 1 mM Bis-Tris buffer, pH 6.3 Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDPglucose + glucose 6-phosphate
-
Saccharomyces cerevisiae trehalose 6-phosphate + UDP
-
?
UDPglucose + glucose 6-phosphate key enzyme for biosynthesis of trehalose, the major soluble carbohydrate in resting cells Saccharomyces cerevisiae trehalose 6-phosphate + UDP
-
?
UDPglucose + glucose 6-phosphate
-
Saccharomyces cerevisiae C13-ABYS86 trehalose 6-phosphate + UDP
-
?
UDPglucose + glucose 6-phosphate key enzyme for biosynthesis of trehalose, the major soluble carbohydrate in resting cells Saccharomyces cerevisiae C13-ABYS86 trehalose 6-phosphate + UDP
-
?

Subunits

Subunits Comment Organism
? x * 56200, calculation from nucleotide sequence Saccharomyces cerevisiae
? x * 55000, isozyme TPS1, x * 100000, isozyme TPS2, x * 105000, isozyme TPS3, SDS-PAGE Saccharomyces cerevisiae