General Stability | Organism |
---|---|
stabilization during purification, 10fold by addition of 20 mM MgCl2, 50 mM NaCl, 2 M glycine betaine and 5 mM mercaptoethanol | Halorhodospira halochloris |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
trehalose | non-competitive | Halorhodospira halochloris |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.5 | - |
UDPglucose | - |
Halorhodospira halochloris | |
2 | - |
glucose 6-phosphate | - |
Halorhodospira halochloris |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Halorhodospira halochloris | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
KHCO3 | 100-300 mM, activation | Halorhodospira halochloris | |
Mg2+ | optimal concentration: 3-6 mM MgCl2 | Halorhodospira halochloris | |
NaHCO3 | 100-300 mM, activation | Halorhodospira halochloris |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
63000 | - |
gel filtration | Halorhodospira halochloris |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Halorhodospira halochloris | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial | Halorhodospira halochloris |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
UDP-alpha-D-glucose + D-glucose 6-phosphate = UDP + alpha,alpha-trehalose 6-phosphate | ordered bi-bi mechanism | Halorhodospira halochloris |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.127 | - |
UDPglucose | Halorhodospira halochloris |
Storage Stability | Organism |
---|---|
-18°C, 2-3 d, no loss of activity | Halorhodospira halochloris |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDPglucose + glucose 6-phosphate | specific for UDPglucose, no reaction with ADPglucose or GDPglucose | Halorhodospira halochloris | trehalose 6-phosphate + UDP | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Halorhodospira halochloris |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
potassium phosphate buffer | Halorhodospira halochloris |
7 | - |
Tris/HCl buffer | Halorhodospira halochloris |