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Literature summary for 2.4.1.144 extracted from
- Expression of bisecting N-acetylglucosaminyltransferase-III in human hepatocarcinoma tissues, fetal liver tissues, and hepatoma cell lines of Hep3B and HepG2 (2001), Cancer Invest., 19, 799-807.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| deoxymannojirimycin | increase in activity, HepG2 and Hep3B cells | Homo sapiens |  |
| additional information | not affected by swainsonine | Homo sapiens | |
| Triton X-100 | - |
Homo sapiens | |
| tunicamycin | increase in activity, HepG2 and Hep3B cells | Homo sapiens | |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| cloning of cDNA by RT-PCR | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.1 | - |
pyridylaminated acceptor substrate | Hep3B cells | Homo sapiens | |
| 3.4 | - |
pyridylaminated acceptor substrate | HepG2 cells | Homo sapiens | |
| 4.7 | - |
UDP-N-acetyl-D-glucosamine | Hep3B cells | Homo sapiens | |
| 6.8 | - |
UDP-N-acetyl-D-glucosamine | HepG2 cells | Homo sapiens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | minor fraction | Homo sapiens | 5829 | - |
| membrane | - |
Homo sapiens | 16020 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mn2+ | HepG2 cells: maximal at 12 mM, Hep3B cells: maximal at 15 mM | Homo sapiens | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| HEP-3B cell | hepatoma cell line | Homo sapiens | - |
| Hep-G2 cell | hepatoma cell line | Homo sapiens | - |
| hepatocyte | primary | Homo sapiens | - |
| hepatocyte | very low amount | Homo sapiens | - |
| hepatocyte | different expression level in fetal and adult hepatocytes | Homo sapiens | - |
| hepatoma cell | - |
Homo sapiens | - |
| liver | very low amount | Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.00000013 | - |
normal liver | Homo sapiens |
| 0.0000096 | - |
fetal primary hepatocytes | Homo sapiens |
| 0.0000124 | - |
hepatic carcinoma cells | Homo sapiens |
| 0.000018 | - |
HepG2 cells | Homo sapiens |
| 0.000024 | - |
Hep3B cells | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-N-acetyl-D-glucosamine + (N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6)-beta-D-mannosyl-1,4-N-acetyl-beta-D-glucosaminyl-R | pyridylaminated biantennary sugar chain | Homo sapiens | UDP + N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6)-(N-acetyl-beta-D-glucosaminyl-1,4)-beta-D-mannosyl-1,4-N-acetyl-beta-D-glucosaminyl-R | - |
ir | |
| UDP-N-acetyl-D-glucosamine + pyridylaminated acceptor substrate | - |
Homo sapiens | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| GlcNAc-transferase-III | - |
Homo sapiens |
| uridine diphosphate (UDP)-N-acetylglucosamin/beta-D-mannoside beta-1,4-N-acetylglucosaminyltransferase III | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
HepG2 cells | Homo sapiens |
| 43 | - |
Hep3B cells | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
HepG2 cells | Homo sapiens |
| 6.5 | - |
Hep3B cells | Homo sapiens |
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