Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutants in COS-1 cells | Rattus norvegicus |
Protein Variants | Comment | Organism |
---|---|---|
N243Q | site-directed mutagenesis of 1 N-glycosylation site, 40-50% activity compared to the wild-type, slightly increased Km-value for the donor substrate | Rattus norvegicus |
N243Q/N261Q | site-directed mutagenesis of 2 N-glycosylation sites, 10% activity compared to the wild-type | Rattus norvegicus |
N243Q/N261Q/N399Q | site-directed mutagenesis of all N-glycosylation sites, no remaining activity | Rattus norvegicus |
N243Q/N399Q | site-directed mutagenesis of 2 N-glycosylation sites, highly reduced activity | Rattus norvegicus |
N261Q | site-directed mutagenesis of 1 N-glycosylation site, 40-50% activity compared to the wild-type, slightly increased Km-value for the donor substrate | Rattus norvegicus |
N261Q/N399Q | site-directed mutagenesis of 2 N-glycosylation sites, highly reduced activity | Rattus norvegicus |
N399Q | site-directed mutagenesis of 1 N-glycosylation site, 70-80% activity compared to the wild-type | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
castanospermine | activity is not affected, but the enzyme is not localized in the Golgi apparatus | Rattus norvegicus | |
tunicamycin | completely abolishes the enzyme activity due to deglycosylation of the enzyme, not localized in the Golgi apparatus | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
endoplasmic reticulum | tunicamycin-treated and castanospermine-treated enzyme, not native enzyme | Rattus norvegicus | 5783 | - |
Golgi apparatus | deglycosylated mutants are not localized in the Golgi apparatus | Rattus norvegicus | 5794 | - |
Golgi apparatus | wild-type | Rattus norvegicus | 5794 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
68100 | - |
x * 68100, wild-type enzyme, SDS-PAGE | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | requires core glycosylation but not glucose trimming | Rattus norvegicus |
glycoprotein | 3 N-glycosylation sites: Asn243, Asn261, Asn399, essential for activity, tunicamycin-treated enzyme, which is not glycosylated, shows no activity | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-N-acetyl-D-glucosamine + (N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6)-beta-D-mannosyl-1,4-N-acetyl-beta-D-glucosaminyl-R | - |
Rattus norvegicus | UDP + N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6)-(N-acetyl-beta-D-glucosaminyl-1,4)-beta-D-mannosyl-1,4-N-acetyl-beta-D-glucosaminyl-R | - |
? | |
UDP-N-acetyl-D-glucosamine + GlcNAc-beta-1,2-Man-alpha-1,6-(GlcNAc-beta-1,2-Man-alpha-1,3)-Man-beta-1,4-GlcNAc-beta-1,4-GlcNAc-2-aminopyridine | - |
Rattus norvegicus | UDP + GlcNAc-beta-1,2-Man-alpha-1,6-(GlcNAc-beta-1,2-Man-alpha-1,3)(GlcNAc-beta-1,4)-Man-beta-1,4-GlcNAc-beta-1,4-GlcNAc-2-aminopyridine | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 68100, wild-type enzyme, SDS-PAGE | Rattus norvegicus |
Synonyms | Comment | Organism |
---|---|---|
GnT-III | - |
Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Rattus norvegicus |