Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Triton X-100 | activation, 0.1% | Vigna radiata var. radiata |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0166 | - |
alpha-D-mannosyl-1,6-(N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetylglucosaminyl-R | pH 6.5 | Vigna radiata var. radiata | |
0.018 | - |
UDP-N-acetylglucosamine | pH 6.5 | Vigna radiata var. radiata |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Vigna radiata var. radiata | 16020 | - |
microsome | - |
Vigna radiata var. radiata | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cd2+ | activation, can replace Mn2+ to some extent, 0.5-1 mM | Vigna radiata var. radiata | |
Mn2+ | requirement | Vigna radiata var. radiata | |
Mn2+ | 2-3 mM | Vigna radiata var. radiata | |
additional information | Ca2+, Co2+, Cu2+, Hg2+, Mg2+ or Zn2+ cannot replace Mn2+, at 1 or 2.5 mM each | Vigna radiata var. radiata |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Vigna radiata var. radiata | - |
mung bean | - |
Purification (Comment) | Organism |
---|---|
solubilized with Triton X-100 | Vigna radiata var. radiata |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
seedling | - |
Vigna radiata var. radiata | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
9.3 | - |
- |
Vigna radiata var. radiata |
Storage Stability | Organism |
---|---|
-20°C, several weeks in 20% glycerol, 0.0005 mM DTT and 0.1% Triton X-100 | Vigna radiata var. radiata |
0°C, at least 1 week | Vigna radiata var. radiata |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-N-acetyl-D-glucosamine + alpha-D-mannosyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetylglucosaminyl-R | best acceptor, high specificity | Vigna radiata var. radiata | UDP + N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetylglucosaminyl-R | - |
? | |
UDP-N-acetyl-D-glucosamine + alpha-D-mannosyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetylglucosaminyl-R | UDP-N-acetyl-D-glucosamine cannot be replaced by UDP-N-acetylgalactosamine, UDPgalactose, UDPxylose, UDPglucose or GDPfucose | Vigna radiata var. radiata | UDP + N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetylglucosaminyl-R | - |
? | |
UDP-N-acetyl-D-glucosamine + alpha-D-mannosyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetylglucosaminyl-R | no acceptor substrates: overview | Vigna radiata var. radiata | UDP + N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetylglucosaminyl-R | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | 7 | - |
Vigna radiata var. radiata |