Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.4.1.143 extracted from

  • Szumilo, T.; Kaushal, G.P.; Elbein, A.D.
    Purification and properties of the glycoprotein processing N-acetylglucosaminyltransferase II from plants (1987), Biochemistry, 26, 5498-5505.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
Triton X-100 activation, 0.1% Vigna radiata var. radiata

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0166
-
alpha-D-mannosyl-1,6-(N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetylglucosaminyl-R pH 6.5 Vigna radiata var. radiata
0.018
-
UDP-N-acetylglucosamine pH 6.5 Vigna radiata var. radiata

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
Vigna radiata var. radiata 16020
-
microsome
-
Vigna radiata var. radiata
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Cd2+ activation, can replace Mn2+ to some extent, 0.5-1 mM Vigna radiata var. radiata
Mn2+ requirement Vigna radiata var. radiata
Mn2+ 2-3 mM Vigna radiata var. radiata
additional information Ca2+, Co2+, Cu2+, Hg2+, Mg2+ or Zn2+ cannot replace Mn2+, at 1 or 2.5 mM each Vigna radiata var. radiata

Organism

Organism UniProt Comment Textmining
Vigna radiata var. radiata
-
mung bean
-

Purification (Commentary)

Purification (Comment) Organism
solubilized with Triton X-100 Vigna radiata var. radiata

Source Tissue

Source Tissue Comment Organism Textmining
seedling
-
Vigna radiata var. radiata
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
9.3
-
-
Vigna radiata var. radiata

Storage Stability

Storage Stability Organism
-20°C, several weeks in 20% glycerol, 0.0005 mM DTT and 0.1% Triton X-100 Vigna radiata var. radiata
0°C, at least 1 week Vigna radiata var. radiata

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-N-acetyl-D-glucosamine + alpha-D-mannosyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetylglucosaminyl-R best acceptor, high specificity Vigna radiata var. radiata UDP + N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetylglucosaminyl-R
-
?
UDP-N-acetyl-D-glucosamine + alpha-D-mannosyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetylglucosaminyl-R UDP-N-acetyl-D-glucosamine cannot be replaced by UDP-N-acetylgalactosamine, UDPgalactose, UDPxylose, UDPglucose or GDPfucose Vigna radiata var. radiata UDP + N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetylglucosaminyl-R
-
?
UDP-N-acetyl-D-glucosamine + alpha-D-mannosyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetylglucosaminyl-R no acceptor substrates: overview Vigna radiata var. radiata UDP + N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetylglucosaminyl-R
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 7
-
Vigna radiata var. radiata