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Literature summary for 2.4.1.143 extracted from

  • Bendiak, B.; Schachter, H.
    Control of glycoprotein synthesis. Purification of UDP-N-acetylglucosamine:alpha-D-mannoside beta 1-2 N-acetylglucosaminyltransferase II from rat liver (1987), J. Biol. Chem., 262, 5775-5783.
    View publication on PubMed
Search for more literature for 2.4.1.143

General Stability

General Stability Organism
Triton X-100 stabilizes Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
5-Hg-UDP reversible Rattus norvegicus
5-Hg-UDP-N-acetylglucosamine reversible Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Rattus norvegicus 16020
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
 rat enzyme in crude extract exists in 2 forms, high-molecular weight form: above MW 200000, low-molecular weight form: MW 43000-48000, gel filtration Rattus norvegicus
43000
-
 x * 43000, x * 48000, SDS-PAGE, one or both of these bands represent the enzyme Rattus norvegicus
48000
-
 x * 43000, x * 48000, SDS-PAGE, one or both of these bands represent the enzyme Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-N-acetyl-D-glucosamine + alpha-D-mannosyl-1,6-(N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetylglucosaminyl-R Rattus norvegicus essential for biosynthesis of complex N-linked oligosaccharides UDP + N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetylglucosaminyl-R
-
 ?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
solubilized with Triton X-100/NaCl, enzyme in crude extract exists in 2 molecular weight forms: purification of low-molecular weight form, affinity chromatography on Hg-UDP-N-acetylglucosamine-thiopropyl-Sepharose Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
27.5
-
-
 Rattus norvegicus

Storage Stability

Storage Stability Organism
4°C, at least 6 months in 20% glycerol, 10 mM EDTA and 0.1% Triton X-100 Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-N-acetyl-D-glucosamine + alpha-D-mannosyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetylglucosaminyl-R R represents the remainder of the N-oligosaccharide core (n fucose), R: H or 1,4-(+/-)-fucose-1,6-N-acetylglucosaminyl-1-N-asparagine Rattus norvegicus UDP + N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetylglucosaminyl-R
-
 ?
UDP-N-acetyl-D-glucosamine + alpha-D-mannosyl-1,6-(N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetylglucosaminyl-R essential for biosynthesis of complex N-linked oligosaccharides Rattus norvegicus UDP + N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetylglucosaminyl-R
-
 ?

Subunits

Subunits Comment Organism
? x * 43000, x * 48000, SDS-PAGE, one or both of these bands represent the enzyme Rattus norvegicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Rattus norvegicus