Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.4.1.133 extracted from

  • Garcia-Garcia, J.F.; Corrales, G.; Casas, J.; Fernandez-Mayoralas, A.; Garcia-Junceda, E.
    Synthesis and evaluation of xylopyranoside derivatives as decoy acceptors of human beta-1,4-galactosyltransferase 7 (2011), Mol. Biosyst., 7, 1312-1321.
    View publication on PubMed
Search for more literature for 2.4.1.133

Cloned(Commentary)

Cloned (Comment) Organism
truncated enzyme form is expressed in Escherichia coli BL21(DE3) cells Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
2-(6-hydroxynaphthyl) beta-D-xylopyranoside
-
 Homo sapiens
3-acetamido-propyl beta-D-xylopyranoside
-
 Homo sapiens
3-benzamido-propyl beta-D-xylopyranoside
-
 Homo sapiens
3-hexanamido-propyl beta-D-xylopyranoside
-
 Homo sapiens
N-[O-(beta-D-xylopyranosyl)-3-hydroxypropyl]phthalamate
-
 Homo sapiens
N-[O-(beta-D-xylopyranosyl)-3-hydroxypropyl]succinamate
-
 Homo sapiens
O-gamma-beta-D-xylopyranosyl-N-Cbz-L-serine methyl ester
-
 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.4
-
 N-[O-(beta-D-xylopyranosyl)-3-hydroxypropyl]phthalamate recombinant enzyme, in sodium acetate buffer (25 mM, pH 5.0), 20 mM MnCl2, 50 mM KCl, at 25°C Homo sapiens
0.53
-
 3-benzamido-propyl beta-D-xylopyranoside recombinant enzyme, in sodium acetate buffer (25 mM, pH 5.0), 20 mM MnCl2, 50 mM KCl, at 25°C Homo sapiens
0.53
-
 O-gamma-beta-D-xylopyranosyl-N-Cbz-L-serine methyl ester recombinant enzyme, in sodium acetate buffer (25 mM, pH 5.0), 20 mM MnCl2, 50 mM KCl, at 25°C Homo sapiens
0.56
-
 N-[O-(beta-D-xylopyranosyl)-3-hydroxypropyl]succinamate recombinant enzyme, in sodium acetate buffer (25 mM, pH 5.0), 20 mM MnCl2, 50 mM KCl, at 25°C Homo sapiens
0.58
-
 2-(6-hydroxynaphthyl) beta-D-xylopyranoside recombinant enzyme, in sodium acetate buffer (25 mM, pH 5.0), 20 mM MnCl2, 50 mM KCl, at 25°C Homo sapiens
0.75
-
 3-acetamido-propyl beta-D-xylopyranoside recombinant enzyme, in sodium acetate buffer (25 mM, pH 5.0), 20 mM MnCl2, 50 mM KCl, at 25°C Homo sapiens
0.75
-
 3-hexanamido-propyl beta-D-xylopyranoside recombinant enzyme, in sodium acetate buffer (25 mM, pH 5.0), 20 mM MnCl2, 50 mM KCl, at 25°C Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Homo sapiens 16020
-

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein the human enzyme presents only one potential glycosylation site at Asn154 Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
nickel-iminodiacetic acid column chromatography Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-galactose + 2-(6-hydroxynaphthyl) beta-D-xylopyranoside
-
 Homo sapiens ?
-
 ?
UDP-galactose + 3-acetamido-propyl beta-D-xylopyranoside
-
 Homo sapiens ?
-
 ?
UDP-galactose + 3-benzamido-propyl beta-D-xylopyranoside
-
 Homo sapiens ?
-
 ?
UDP-galactose + 3-hexanamido-propyl beta-D-xylopyranoside
-
 Homo sapiens ?
-
 ?
UDP-galactose + 4-nitrophenyl O-beta-D-xylopyranoside
-
 Homo sapiens UDP + 4-nitrophenyl 4-O-beta-D-galactopyranosyl-beta-D-xylopyranoside
-
 ?
UDP-galactose + N-[O-(beta-D-xylopyranosyl)-3-hydroxypropyl]phthalamate
-
 Homo sapiens ?
-
 ?
UDP-galactose + N-[O-(beta-D-xylopyranosyl)-3-hydroxypropyl]succinamate
-
 Homo sapiens ?
-
 ?
UDP-galactose + O-gamma-2,3,4-tri-O-acetyl-(beta-D-xylopyranosyl)-N-Cbz-L-serine methyl ester
-
 Homo sapiens ?
-
 ?
UDP-galactose + O-gamma-beta-D-xylopyranosyl-N-Cbz-L-serine methyl ester
-
 Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
beta-1,4-galactosyltransferase
-
 Homo sapiens
beta-1,4-GalT 7
-
 Homo sapiens
UDP-galactose:O-beta-D-xylosylprotein 4-beta-D-galactosyltransferase
-
 Homo sapiens
xylosylprotein 4-beta-galactosyltransferase
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
30.2
-
 3-acetamido-propyl beta-D-xylopyranoside recombinant enzyme, in sodium acetate buffer (25 mM, pH 5.0), 20 mM MnCl2, 50 mM KCl, at 25°C Homo sapiens
41
-
 3-hexanamido-propyl beta-D-xylopyranoside recombinant enzyme, in sodium acetate buffer (25 mM, pH 5.0), 20 mM MnCl2, 50 mM KCl, at 25°C Homo sapiens
47.2
-
 3-benzamido-propyl beta-D-xylopyranoside recombinant enzyme, in sodium acetate buffer (25 mM, pH 5.0), 20 mM MnCl2, 50 mM KCl, at 25°C Homo sapiens
64.3
-
 N-[O-(beta-D-xylopyranosyl)-3-hydroxypropyl]succinamate recombinant enzyme, in sodium acetate buffer (25 mM, pH 5.0), 20 mM MnCl2, 50 mM KCl, at 25°C Homo sapiens
78.7
-
 N-[O-(beta-D-xylopyranosyl)-3-hydroxypropyl]phthalamate recombinant enzyme, in sodium acetate buffer (25 mM, pH 5.0), 20 mM MnCl2, 50 mM KCl, at 25°C Homo sapiens
106.3
-
 O-gamma-beta-D-xylopyranosyl-N-Cbz-L-serine methyl ester recombinant enzyme, in sodium acetate buffer (25 mM, pH 5.0), 20 mM MnCl2, 50 mM KCl, at 25°C Homo sapiens
152
-
 2-(6-hydroxynaphthyl) beta-D-xylopyranoside recombinant enzyme, in sodium acetate buffer (25 mM, pH 5.0), 20 mM MnCl2, 50 mM KCl, at 25°C Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.3
-
 2-(6-hydroxynaphthyl) beta-D-xylopyranoside recombinant enzyme, in sodium acetate buffer (25 mM, pH 5.0), 20 mM MnCl2, 50 mM KCl, at 25°C Homo sapiens
0.61
-
 N-[O-(beta-D-xylopyranosyl)-3-hydroxypropyl]phthalamate recombinant enzyme, in sodium acetate buffer (25 mM, pH 5.0), 20 mM MnCl2, 50 mM KCl, at 25°C Homo sapiens
1.04
-
 3-benzamido-propyl beta-D-xylopyranoside recombinant enzyme, in sodium acetate buffer (25 mM, pH 5.0), 20 mM MnCl2, 50 mM KCl, at 25°C Homo sapiens
2.39
-
 O-gamma-beta-D-xylopyranosyl-N-Cbz-L-serine methyl ester recombinant enzyme, in sodium acetate buffer (25 mM, pH 5.0), 20 mM MnCl2, 50 mM KCl, at 25°C Homo sapiens
2.46
-
 3-hexanamido-propyl beta-D-xylopyranoside recombinant enzyme, in sodium acetate buffer (25 mM, pH 5.0), 20 mM MnCl2, 50 mM KCl, at 25°C Homo sapiens
3.69
-
 3-acetamido-propyl beta-D-xylopyranoside recombinant enzyme, in sodium acetate buffer (25 mM, pH 5.0), 20 mM MnCl2, 50 mM KCl, at 25°C Homo sapiens
3.96
-
 N-[O-(beta-D-xylopyranosyl)-3-hydroxypropyl]succinamate recombinant enzyme, in sodium acetate buffer (25 mM, pH 5.0), 20 mM MnCl2, 50 mM KCl, at 25°C Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
52.1
-
 2-(6-hydroxynaphthyl) beta-D-xylopyranoside recombinant enzyme, in sodium acetate buffer (25 mM, pH 5.0), 20 mM MnCl2, 50 mM KCl, at 25°C Homo sapiens
54.7
-
 3-acetamido-propyl beta-D-xylopyranoside recombinant enzyme, in sodium acetate buffer (25 mM, pH 5.0), 20 mM MnCl2, 50 mM KCl, at 25°C Homo sapiens
84.3
-
 N-[O-(beta-D-xylopyranosyl)-3-hydroxypropyl]succinamate recombinant enzyme, in sodium acetate buffer (25 mM, pH 5.0), 20 mM MnCl2, 50 mM KCl, at 25°C Homo sapiens
85.7
-
 3-hexanamido-propyl beta-D-xylopyranoside recombinant enzyme, in sodium acetate buffer (25 mM, pH 5.0), 20 mM MnCl2, 50 mM KCl, at 25°C Homo sapiens
148.5
-
 3-benzamido-propyl beta-D-xylopyranoside recombinant enzyme, in sodium acetate buffer (25 mM, pH 5.0), 20 mM MnCl2, 50 mM KCl, at 25°C Homo sapiens
265.7
-
 N-[O-(beta-D-xylopyranosyl)-3-hydroxypropyl]phthalamate recombinant enzyme, in sodium acetate buffer (25 mM, pH 5.0), 20 mM MnCl2, 50 mM KCl, at 25°C Homo sapiens
286.8
-
 O-gamma-beta-D-xylopyranosyl-N-Cbz-L-serine methyl ester recombinant enzyme, in sodium acetate buffer (25 mM, pH 5.0), 20 mM MnCl2, 50 mM KCl, at 25°C Homo sapiens