Cloned (Comment) | Organism |
---|---|
expressed as a GST-fusion protein in Escherichia coli and as Myc-tagged fusion proteins in HeLa cells | Homo sapiens |
expressed in Escherichia coli BL21(DE3) cells, HeLa cells and CHO pgsB-618 cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
D163A | inactive | Homo sapiens |
D165A | inactive | Homo sapiens |
D165E | the apparent Km values of the D165E mutant toward UDP-galactose and 4-methylumbelliferyl-O-beta-D-xylopyranoside are about 3.8 and 5.5fold, respectively, higher than the wild type enzyme | Homo sapiens |
D228A | inactive | Homo sapiens |
D228E | the mutant expressed in HeLa cells retains about 40% activity of the wild type enzyme | Homo sapiens |
D230A | the mutant shows increased Km values towards UDP-galactose and 4-methylumbelliferyl-O-beta-D-xylopyranoside compared to the wild type enzyme | Homo sapiens |
E227D | the mutant displays similar apparent Km values toward both donor and acceptor substrates compared with the wild type enzyme | Homo sapiens |
F221A | the mutant shows increased Km values towards UDP-galactose and 4-methylumbelliferyl-O-beta-D-xylopyranoside compared to the wild type enzyme | Homo sapiens |
F221Y | the mutant shows increased Km values towards UDP-galactose and 4-methylumbelliferyl-O-beta-D-xylopyranoside compared to the wild type enzyme | Homo sapiens |
G223a | the mutant is about 40% less active than the wild type enzyme | Homo sapiens |
G225A | the mutant does not display any in vitro activity | Homo sapiens |
V164A | the mutant shows increased Km values towards UDP-galactose and 4-methylumbelliferyl-O-beta-D-xylopyranoside compared to the wild type enzyme | Homo sapiens |
W222A | the mutant shows decreased Km value towards UDP-galactose compared to the wild type enzyme | Homo sapiens |
W222F | the mutation has no significant effect on the Km value toward donor or acceptor substrate | Homo sapiens |
W224H | the mutant is able to sustain decorin glycosaminoglycan chain substitution but not glycosaminoglycan synthesis from exogenously added xyloside | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
UDP-galactose | pH 7.0, 37°C, mutant W224H, protein expressed in Escherichia coli | Homo sapiens | |
0.07 | - |
UDP-galactose | pH 7.0, 37°C, mutant W224H, protein expressed in HeLa cells | Homo sapiens | |
0.07 | - |
UDP-galactose | mutant enzyme W224H, in 100 mM sodium cacodylate buffer (pH 7.0) containing 10 mM MnCl2, at 37°C | Homo sapiens | |
0.1 | - |
UDP-galactose | pH 7.0, 37°C, mutant E227D, protein expressed in Escherichia coli | Homo sapiens | |
0.16 | - |
4-methylumbelliferyl-beta-D-xylopyranoside | pH 7.0, 37°C, mutant W222F, protein expressed in HeLa cells | Homo sapiens | |
0.21 | - |
UDP-galactose | pH 7.0, 37°C, mutant W222A, protein expressed in HeLa cells | Homo sapiens | |
0.21 | - |
UDP-galactose | mutant enzyme W222A, in 100 mM sodium cacodylate buffer (pH 7.0) containing 10 mM MnCl2, at 37°C | Homo sapiens | |
0.28 | - |
UDP-galactose | pH 7.0, 37°C, wild-type, protein expressed in Escherichia coli | Homo sapiens | |
0.31 | - |
UDP-galactose | pH 7.0, 37°C, wild-type, protein expressed in HeLa cells | Homo sapiens | |
0.31 | - |
UDP-galactose | wild type enzyme, in 100 mM sodium cacodylate buffer (pH 7.0) containing 10 mM MnCl2, at 37°C | Homo sapiens | |
0.32 | - |
4-methylumbelliferyl-beta-D-xylopyranoside | pH 7.0, 37°C, wild-type, protein expressed in HeLa cells | Homo sapiens | |
0.32 | - |
4-methylumbelliferyl O-beta-D-xylopyranoside | wild type enzyme, in 100 mM sodium cacodylate buffer (pH 7.0) containing 10 mM MnCl2, at 37°C | Homo sapiens | |
0.33 | - |
UDP-galactose | pH 7.0, 37°C, mutant F221Y, protein expressed in HeLa cells | Homo sapiens | |
0.33 | - |
4-methylumbelliferyl-beta-D-xylopyranoside | pH 7.0, 37°C, wild-type, protein expressed in Escherichia coli | Homo sapiens | |
0.33 | - |
UDP-galactose | mutant enzyme F221Y, in 100 mM sodium cacodylate buffer (pH 7.0) containing 10 mM MnCl2, at 37°C | Homo sapiens | |
0.36 | - |
UDP-galactose | pH 7.0, 37°C, mutant F221A, protein expressed in HeLa cells | Homo sapiens | |
0.36 | - |
UDP-galactose | mutant enzyme F221A, in 100 mM sodium cacodylate buffer (pH 7.0) containing 10 mM MnCl2, at 37°C | Homo sapiens | |
0.38 | - |
4-methylumbelliferyl-beta-D-xylopyranoside | pH 7.0, 37°C, mutant F221Y, protein expressed in HeLa cells | Homo sapiens | |
0.38 | - |
4-methylumbelliferyl O-beta-D-xylopyranoside | mutant enzyme F221Y, in 100 mM sodium cacodylate buffer (pH 7.0) containing 10 mM MnCl2, at 37°C | Homo sapiens | |
0.43 | - |
UDP-galactose | pH 7.0, 37°C, mutant D230A, protein expressed in HeLa cells | Homo sapiens | |
0.43 | - |
UDP-galactose | mutant enzyme D230A, in 100 mM sodium cacodylate buffer (pH 7.0) containing 10 mM MnCl2, at 37°C | Homo sapiens | |
0.45 | - |
UDP-galactose | pH 7.0, 37°C, mutant G223A, protein expressed in HeLa cells | Homo sapiens | |
0.45 | - |
UDP-galactose | pH 7.0, 37°C, mutant W222F, protein expressed in HeLa cells | Homo sapiens | |
0.45 | - |
UDP-galactose | mutant enzyme G223A, in 100 mM sodium cacodylate buffer (pH 7.0) containing 10 mM MnCl2, at 37°C | Homo sapiens | |
0.45 | - |
UDP-galactose | mutant enzyme W222F, in 100 mM sodium cacodylate buffer (pH 7.0) containing 10 mM MnCl2, at 37°C | Homo sapiens | |
0.47 | - |
UDP-galactose | pH 7.0, 37°C, mutant D228E, protein expressed in HeLa cells | Homo sapiens | |
0.47 | - |
UDP-galactose | pH 7.0, 37°C, mutant E227D, protein expressed in HeLa cells | Homo sapiens | |
0.47 | - |
UDP-galactose | mutant enzyme D228E, in 100 mM sodium cacodylate buffer (pH 7.0) containing 10 mM MnCl2, at 37°C | Homo sapiens | |
0.47 | - |
UDP-galactose | mutant enzyme E227D, in 100 mM sodium cacodylate buffer (pH 7.0) containing 10 mM MnCl2, at 37°C | Homo sapiens | |
0.48 | - |
UDP-galactose | pH 7.0, 37°C, mutant V164A, protein expressed in HeLa cells | Homo sapiens | |
0.48 | - |
UDP-galactose | mutant enzyme V164A, in 100 mM sodium cacodylate buffer (pH 7.0) containing 10 mM MnCl2, at 37°C | Homo sapiens | |
0.52 | - |
4-methylumbelliferyl-beta-D-xylopyranoside | pH 7.0, 37°C, mutant E227D, protein expressed in HeLa cells | Homo sapiens | |
0.53 | - |
4-methylumbelliferyl-beta-D-xylopyranoside | pH 7.0, 37°C, mutant V164A, protein expressed in HeLa cells | Homo sapiens | |
0.53 | - |
4-methylumbelliferyl O-beta-D-xylopyranoside | mutant enzyme V164A, in 100 mM sodium cacodylate buffer (pH 7.0) containing 10 mM MnCl2, at 37°C | Homo sapiens | |
0.57 | - |
4-methylumbelliferyl-beta-D-xylopyranoside | pH 7.0, 37°C, mutant D230A, protein expressed in HeLa cells | Homo sapiens | |
0.73 | - |
4-methylumbelliferyl-beta-D-xylopyranoside | pH 7.0, 37°C, mutant G223A, protein expressed in HeLa cells | Homo sapiens | |
0.74 | - |
4-methylumbelliferyl-beta-D-xylopyranoside | pH 7.0, 37°C, mutant E227D, protein expressed in Escherichia coli | Homo sapiens | |
1.17 | - |
UDP-galactose | pH 7.0, 37°C, mutant D165E, protein expressed in HeLa cells | Homo sapiens | |
1.17 | - |
UDP-galactose | mutant enzyme D165E, in 100 mM sodium cacodylate buffer (pH 7.0) containing 10 mM MnCl2, at 37°C | Homo sapiens | |
1.76 | - |
4-methylumbelliferyl-beta-D-xylopyranoside | pH 7.0, 37°C, mutant D165E, protein expressed in HeLa cells | Homo sapiens | |
1.76 | - |
4-methylumbelliferyl O-beta-D-xylopyranoside | mutant enzyme D165E, in 100 mM sodium cacodylate buffer (pH 7.0) containing 10 mM MnCl2, at 37°C | Homo sapiens | |
2.57 | - |
4-methylumbelliferyl-beta-D-xylopyranoside | pH 7.0, 37°C, mutant D228E, protein expressed in HeLa cells | Homo sapiens | |
4 | - |
4-methylumbelliferyl-beta-D-xylopyranoside | pH 7.0, 37°C, mutant W224H, value above 4, protein expressed in HeLa cells | Homo sapiens | |
4.22 | - |
4-methylumbelliferyl-beta-D-xylopyranoside | pH 7.0, 37°C, mutant F221A, protein expressed in HeLa cells | Homo sapiens | |
4.22 | - |
4-methylumbelliferyl O-beta-D-xylopyranoside | mutant enzyme F221A, in 100 mM sodium cacodylate buffer (pH 7.0) containing 10 mM MnCl2, at 37°C | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q9UBV7 | - |
- |
Purification (Comment) | Organism |
---|---|
glutathione-Sepharose column chromatography | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-galactose + 4-methylumbelliferyl O-beta-D-xylopyranoside | - |
Homo sapiens | UDP + 4-methylumbelliferyl 4-O-beta-D-galactopyranosyl-beta-D-xylopyranoside | - |
? | |
UDP-galactose + 4-methylumbelliferyl-beta-D-xylopyranoside | - |
Homo sapiens | ? | - |
? | |
UDP-galactose + 4-nitrophenyl O-beta-D-xylopyranoside | - |
Homo sapiens | UDP + 4-nitrophenyl 4-O-beta-D-galactopyranosyl-beta-D-xylopyranoside | - |
? |
Synonyms | Comment | Organism |
---|---|---|
beta1,4-galactosyltransferase 7 | - |
Homo sapiens |
beta4GalT7 | - |
Homo sapiens |
xylosylprotein beta1,4-galactosyltransferase | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
UDP-galactose | pH 7.0, 37°C, mutant W224H, protein expressed in Escherichia coli | Homo sapiens | |
0.13 | - |
4-methylumbelliferyl-beta-D-xylopyranoside | pH 7.0, 37°C, mutant E227D, protein expressed in Escherichia coli | Homo sapiens | |
0.46 | - |
UDP-galactose | pH 7.0, 37°C, mutant E227D, protein expressed in Escherichia coli | Homo sapiens | |
1.52 | - |
UDP-galactose | pH 7.0, 37°C, wild-type, protein expressed in Escherichia coli | Homo sapiens | |
1.79 | - |
4-methylumbelliferyl-beta-D-xylopyranoside | pH 7.0, 37°C, wild-type, protein expressed in Escherichia coli | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Homo sapiens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.17 | - |
4-methylumbelliferyl-beta-D-xylopyranoside | pH 7.0, 37°C, mutant E227D, protein expressed in Escherichia coli | Homo sapiens | |
4.6 | - |
UDP-galactose | pH 7.0, 37°C, mutant E227D, protein expressed in Escherichia coli | Homo sapiens | |
5 | - |
UDP-galactose | pH 7.0, 37°C, mutant W224H, protein expressed in Escherichia coli | Homo sapiens | |
5.42 | - |
4-methylumbelliferyl-beta-D-xylopyranoside | pH 7.0, 37°C, wild-type, protein expressed in Escherichia coli | Homo sapiens | |
5.43 | - |
UDP-galactose | pH 7.0, 37°C, wild-type, protein expressed in Escherichia coli | Homo sapiens |