Literature summary for 2.4.1.13 extracted from

Huang, Y.C.; Hsiang, E.C.; Yang, C.C.; Wang, A.Y.
New insight into the catalytic properties of rice sucrose synthase (2016), Plant Mol. Biol., 90, 127-135.

Search for more literature for 2.4.1.13

Cloned(Commentary)

Cloned (Comment)	Organism
sus3, recombinant expression of wild-type and mutant enzymes in Pichia pastoris	Oryza sativa

Protein Variants

Protein Variants	Comment	Organism
E678D	site-directed mutagenesis, almost inactive mutant	Oryza sativa
E678Q	site-directed mutagenesis, almost inactive mutant, the mutant shows impaired D-fructose binding	Oryza sativa
E686D	site-directed mutagenesis, the mutant enzyme retains 34.9% of sucrose cleavage and 37.9% of sucrose synthesis activity, respectively	Oryza sativa
E686Q	site-directed mutagenesis, inactive mutant, the mutant shows impaired D-fructose binding	Oryza sativa
F680S	site-directed mutagenesis, inactive mutant, the mutant shows impaired D-fructose binding	Oryza sativa
F680Y	site-directed mutagenesis, the mutant enzyme retains 61.0% of sucrose cleavage and all of sucrose synthesis activity, respectively	Oryza sativa

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	required in sucrose synthesis reaction	Oryza sativa

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
336000	375000	recombinant wild-type and mutant enzymes, gel filtration	Oryza sativa

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
UDP-glucose + D-fructose	Oryza sativa	preferred substrates in both reaction directions	UDP + sucrose	-	r

Organism

Organism	UniProt	Comment	Textmining
Oryza sativa	Q43009	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant wild-type and mutant enzymes from Pichia pastris by ammonium sulfate fractionation, anion exchange chromatography, and gel filtration	Oryza sativa

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
UDP-glucose + D-fructose	preferred substrates in both reaction directions	Oryza sativa	UDP + sucrose	-	r
UDP-glucose + D-fructose	the binding of beta-fructopyranose involves residues from both the GT-BN and the GT-BC domains, including those in the E-X7-E motif	Oryza sativa	UDP + sucrose	-	r

Synonyms

Synonyms	Comment	Organism
RSuS3	-	Oryza sativa

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Oryza sativa

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6.4	-	sucrose cleavage	Oryza sativa
7.5	-	sucrose synthesis	Oryza sativa

General Information

General Information	Comment	Organism
evolution	the enzyme belongs to family 4 of the glycosyltransferases (GT4) and contains an E-X7-E motif that is conserved in members of GT4 and two other GT families, sequence comparisons, overview	Oryza sativa
metabolism	key enzyme in sucrose metabolism in higher plants	Oryza sativa
additional information	enzyme homology modeling and substrate docking using the crystal structure of Nitrosomonas europaea NeSuS1, PDB ID 4RBN, and the crystal structure of Arabidopsis AtSuS1, PDB ID 3S28, overview	Oryza sativa
physiological function	the enzyme activity is finely tuned through regulation of their gene expression at different levels and modulation of enzymatic activities by metabolites	Oryza sativa

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Release 2023.1 (January 2023)