Cloned (Comment) | Organism |
---|---|
gene ss2, phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) using plasmid pNESS2 | Nitrosomonas europaea |
Crystallization (Comment) | Organism |
---|---|
purified recombinant His-tagged enzyme, hanging drop vapour diffusion method, mixing of 0.001 ml of 15mg/ml protein solution with 0.001 ml of reservoir solution containing 5% Tacsimate, pH 5.0, 5% w/v PEG 3350, and 0.1 M sodium citrate, pH 5.6, 20°C, 7 days, X-ray diffraction structure determination and analysis at 3.05 A resolution, molecular replacement with Arabidopsis thaliana sucrose synthase structural model, PDB ID 3S29, as a template | Nitrosomonas europaea |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.044 | - |
ADP-glucose | pH 7.0, 60°C | Nitrosomonas europaea | |
0.89 | - |
UDP-glucose | pH 7.0, 60°C | Nitrosomonas europaea | |
5.6 | - |
D-fructose | pH 7.0, 60°C, with ADP-glucose | Nitrosomonas europaea | |
120 | - |
D-fructose | pH 7.0, 60°C, with UDP-glucose | Nitrosomonas europaea |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
95000 | - |
4 * 95000, recombinant His-tagged enzyme, SDS-PAGE, each monomer contains four domains, i.e. SSN-1, SSN-2, GT-B(A), and GT-B(D), structure overview and comparisons | Nitrosomonas europaea |
360000 | - |
recombinant His-tagged enzyme, gel filtration | Nitrosomonas europaea |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP-glucose + D-fructose | Nitrosomonas europaea | - |
ADP + sucrose | - |
r | |
NDP-glucose + D-fructose | Nitrosomonas europaea | - |
NDP + sucrose | - |
r | |
UDP-glucose + D-fructose | Nitrosomonas europaea | - |
UDP + sucrose | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Nitrosomonas europaea | Q820M5 | gene ss2 | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration to homogeneity | Nitrosomonas europaea |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP-glucose + D-fructose | - |
Nitrosomonas europaea | ADP + sucrose | - |
r | |
NDP-glucose + D-fructose | - |
Nitrosomonas europaea | NDP + sucrose | - |
r | |
NDP-glucose + D-fructose | the enzyme prefers transferring glucose from ADP-glucose rather than UDP-glucose | Nitrosomonas europaea | NDP + sucrose | - |
r | |
UDP-glucose + D-fructose | - |
Nitrosomonas europaea | UDP + sucrose | - |
r |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 95000, recombinant His-tagged enzyme, SDS-PAGE, each monomer contains four domains, i.e. SSN-1, SSN-2, GT-B(A), and GT-B(D), structure overview and comparisons | Nitrosomonas europaea |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Nitrosomonas europaea |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Nitrosomonas europaea |
General Information | Comment | Organism |
---|---|---|
evolution | Nitrosomonas europaea sucrose synthase shares the same fold as the GT-B family of the retaining glycosyltransferases | Nitrosomonas europaea |
additional information | a triad of conserved homologous catalytic residues, Arg567, Lys572, and Glu663, in the family is functionally critical in the Nitrosomonas europaea sucrose synthase, structure homology modeling, overview | Nitrosomonas europaea |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.036 | - |
D-fructose | pH 7.0, 60°C, with UDP-glucose | Nitrosomonas europaea | |
1.33 | - |
D-fructose | pH 7.0, 60°C, with ADP-glucose | Nitrosomonas europaea | |
7.5 | - |
ADP-glucose | pH 7.0, 60°C | Nitrosomonas europaea | |
130.3 | - |
UDP-glucose | pH 7.0, 60°C | Nitrosomonas europaea |