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Literature summary for 2.4.1.13 extracted from
- Purification and characterization of sucrose synthase from the cotyledons of Vicia faba L. (1992), Plant Physiol., 100, 1008-1013.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| fructose | - |
Vicia faba |  |
| Tris | ADP + sucrose | Vicia faba | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.14 | 0.15 | UDP | Tris buffer | Vicia faba | |
| 0.17 | 0.2 | GDP | cosubstrate UDP, ADP, HEPES buffer | Vicia faba | |
| 16.9 | 40 | sucrose | - |
Vicia faba | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 92600 | - |
4 * 92600, SDS-PAGE | Vicia faba |
| 353000 | - |
gel filtration | Vicia faba |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Vicia faba | - |
field or faba bean, cv. Maris Bead | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Vicia faba |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cotyledon | - |
Vicia faba | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 8.34 | - |
- |
Vicia faba |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GDP-glucose + D-fructose | - |
Vicia faba | GDP + sucrose | - |
? | |
| UDP + sucrose | also substrate: ADP | Vicia faba | UDP-glucose + D-fructose | - |
? | |
| UDP + sucrose | CDP is poor substrate | Vicia faba | UDP-glucose + D-fructose | - |
? | |
| UDP + sucrose | no substrate: GDP | Vicia faba | UDP-glucose + D-fructose | - |
? | |
| UDP + sucrose | substrate: CDP | Vicia faba | UDP-glucose + D-fructose | - |
? | |
| UDP + sucrose | CDP can replace UDP | Vicia faba | UDP-glucose + D-fructose | - |
? | |
| UDPglucose + D-fructose | - |
Vicia faba | UDP + sucrose | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 92600, SDS-PAGE | Vicia faba |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
pI: 5.4,isozyme SS2 | Vicia faba |
| 6.4 | - |
sucrose cleavage | Vicia faba |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.2 | 6.6 | 96% of maximal activity at pH 6.2 and 6.6 | Vicia faba |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.48 | - |
fructose | - |
Vicia faba | |
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